miniMos
The miniMos reagents are described in: “Frøkjær-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovski A, Moerman DG and Jorgensen EM. Random and targeted transgene insertion in C. elegans using a modified Mos1 transposon. Nature Methods. (In press)”.
The plasmids can be ordered either individually (see links below) or as a kit (29 plasmids – 8 co-injection vectors, 16 cloning vectors, 3 positive controls and 2 LacO vectors) from Addgene. Please see this page for information about ordering the kit. If you plan to order more than 7 plasmids it is cheaper to order the full kit. Addgene is a non-profit plasmid repository and we do not receive any compensation or commission – it is just the easiest way to distribute plasmids and saves us a lot of time dealing with shipping and MTAs.
Co-injection markers
Plasmid | Description | Transgene | ApE file | |
Mos1 transposase | ||||
pCFJ601 | Induces Mos1 transposition by injection. | Peft-3:Mos1 transposase | pCFJ601 | Request plasmid |
pJL44 | Induces Mos1 transposition by heat-shock. | Phsp-16.48:Mos1 transposase | pJL44 | Request plasmid |
Negative selection | ||||
pMA122 | Negative selection, heat-shock inducible. | Phsp-16.41:peel-1:tbb-2 UTR | pMA122 | Request plasmid |
Fluorescent markers | ||||
pGH8 | Visual extra-chromosomal array marker. Red, nervous system, cytosolic. | Prab-3:mCherry:unc-54 UTR | pGH8 | Request plasmid |
pCFJ90 | Visual extra-chromosomal array marker. Red, pharynx, cytosolic. | Pmyo-2:mCherry:unc-54 UTR | pCFJ90 | Request plasmid |
pCFJ104 | Visual extra-chromosomal array marker. Red, body wall muscle, cytosolic. | Pmyo-3:mCherry:unc-54 UTR | pCFJ104 | Request plasmid |
pCFJ420 | Visual extra-chromosomal array marker. Green, ubiquitous, nuclear. | Peft-3:GFP:H2B:tbb-2 UTR | pCFJ420 | Request plasmid |
pCFJ421 | Visual extra-chromosomal array marker. Green, pharynx, nuclear. | Pmyo-2:GFP:H2B:tbb-2 UTR | pCFJ421 | Request plasmid |
miniMos cloning vectors
Positive control vectors + LacO
Plasmid | Transgene | Selection | ApE file (Right click and "save as..") | |
---|---|---|---|---|
Positive controls | ||||
pCFJ914 | Peft-3:GFP:H2B:tbb-2UTR | NeoR | A9 - pCFJ914 - Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR] | Request plasmid |
pCFJ1208 | Peft-3:tdTomato(NLS):tbb-2 UTR | cb-unc-119 | A10 - pCFJ1208 - Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119] | Request plasmid |
pCFJ1209 | Peft-3:tdTomato(NLS):tbb-2 UTR | NeoR | A11 - pCFJ1209 - Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR] | Request plasmid |
256x LacO plasmids | ||||
pCFJ1324 | 256x lacO | cb-unc-119 + NeoR | A12 - pCFJ1324 - Minimal Mos1 [256x LacO, unc-119, NeoR, peel-1] | Request plasmid |
pCFJ1319 | 256x lacO | cb-unc-119 + PuroR | B1 - pCFJ1319 - Minimal Mos1 [256x LacO, unc-119, PuroR, peel-1] | Request plasmid |