Plasmids

MosSCI

Co-injection markers
PlasmidDescriptionTransgeneApE fileAddgene plasmid repository
pCFJ601Induces Mos1 transposition by injection.Peft-3:Mos1 transposasepCFJ601Request plasmid
pJL44Induces Mos1 transposition by heat-shock.Phsp-16.48:Mos1 transposasepJL44Request plasmid
pMA122Negative selection, heat-shock inducible.Phsp-16.41:peel-1:tbb-2 UTRpMA122Request plasmid
pGH8Visual extra-chromosomal array marker. Red, nervous system, cytosolic.Prab-3:mCherry:unc-54 UTRpGH8Request plasmid
pCFJ90Visual extra-chromosomal array marker. Red, pharynx, cytosolic. Pmyo-2:mCherry:unc-54 UTRpCFJ90Request plasmid
pCFJ104Visual extra-chromosomal array marker. Red, body wall muscle, cytosolic.Pmyo-3:mCherry:unc-54 UTRpCFJ104Request plasmid
pCFJ420Visual extra-chromosomal array marker. Green, ubiquitous, nuclear. Peft-3:GFP:H2B:tbb-2 UTRpCFJ420Request plasmid
pCFJ421Visual extra-chromosomal array marker. Green, pharynx, nuclear.Pmyo-2:GFP:H2B:tbb-2 UTRpCFJ421Request plasmid
Site-specific MosSCI vectors
Plasmid Locus Genetic Position Chromosomal Position Ape File Selection Addgene plasmid repository
Three fragment Gateway vectors
pCFJ210ttTi4348 I:-5.32I: 2.85 MBpCFJ210cb-unc-119Request plasmid
pCFJ448ttTi4348 I:-5.32I: 2.85 MBpCFJ448unc-18Request plasmid
pCFJ604ttTi4391I:7.93I: 11.27 MBpCFJ604cb-unc-119Request plasmid
pCFJ150ttTi5605II:0.77II: 8.42 MBpCFJ150cb-unc-119Request plasmid
pCFJ212cxTi10816IV:1.41IV: 5.01 MBpCFJ212cb-unc-119Request plasmid
pCFJ201cxTi10882IV:-0.05IV: 4.24 MBpCFJ201cb-unc-119
pCFJ606ttTi14024X:22.84X: 15.57 MBpCFJ606cb-unc-119Request plasmid
Multiple Cloning Site vectors
pCFJ352ttTi4348 I:-5.32I: 2.85 MBpCFJ352cb-unc-119Request plasmid
pCFJ676ttTi4348 I:-5.32I: 2.85 MBpCFJ676unc-18Request plasmid
pCFJ353ttTi4391I:7.93I: 11.27 MBpCFJ353cb-unc-119Request plasmid
pCFJ350ttTi5605II:0.77II: 8.42 MBpCFJ350cb-unc-119Request plasmid
pCFJ356cxTi10816IV:1.41IV: 5.01 MBpCFJ356cb-unc-119Request plasmid
pCFJ351cxTi10882IV:-0.05IV: 4.24 MBpCFJ351cb-unc-119
pCFJ355ttTi14024X:22.84X: 15.57 MBpCFJ355cb-unc-119Request plasmid