Codon-optimized flourophores

Plasmids described in Froekjaer-Jensen et al (2016). In general, the C. elegans codon optimized fluorophores are better expressed in the germline, especially when the coding region contains PATCs.

PlasmidDescriptionTransgeneApE fileAddgene plasmid repository
pCFJ1955C. elegans optimized tdTomato in expression construct in minimos vectorPeft-3:ceTdTomato(2xNLS):tbb-2 UTRpCFJ1955 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ce-tdTomato(syntrons, NLS) | tbb-2 3UTR].apeRequest plasmid
pCFJ1320C. elegans optimized GFP in expression construct in minimos vectorPeft-3:ceGFP(2xNLS):tbb-2 UTRpCFJ1320 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(smu-2 introns) | tbb-2 3UTR].apeRequest plasmid
pCFJ1415C. elegans optimized GFP with PATCs in expression construct in minimos vectorPeft-3:ceGFP(PATC, 2xNLS):tbb-2 UTRpCFJ1415 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(smu-1 introns) | tbb-2 3UTR].apeRequest plasmid
pCFJ1137C. elegans optimized GFP in expression construct in minimos vectorPeft-3:ceGFP(2xNLS):tbb-2 UTRpCFJ1137 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(synthetic introns) | tbb-2 3UTR.apeRequest plasmid
pCFJ889GFP tagged smu-1 gene in minimos vectorPsmu-1:smu-1:gfp:smu-1UTRpCFJ889 - pEXP(minimos, cbr-unc-119)[Psmu-1 | smu-1| GFP-smu-1 3UTR].apeRequest plasmid