Plasmids described in Froekjaer-Jensen et al (2016). In general, the C. elegans codon optimized fluorophores are better expressed in the germline, especially when the coding region contains PATCs.
| Plasmid | Description | Transgene | ApE file |  |
|---|---|---|---|---|
| pCFJ1955 | C. elegans optimized tdTomato in expression construct in minimos vector | Peft-3:ceTdTomato(2xNLS):tbb-2 UTR | pCFJ1955 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ce-tdTomato(syntrons, NLS) | tbb-2 3UTR].ape | Request plasmid |
| pCFJ1320 | C. elegans optimized GFP in expression construct in minimos vector | Peft-3:ceGFP(2xNLS):tbb-2 UTR | pCFJ1320 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(smu-2 introns) | tbb-2 3UTR].ape | Request plasmid |
| pCFJ1415 | C. elegans optimized GFP with PATCs in expression construct in minimos vector | Peft-3:ceGFP(PATC, 2xNLS):tbb-2 UTR | pCFJ1415 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(smu-1 introns) | tbb-2 3UTR].ape | Request plasmid |
| pCFJ1137 | C. elegans optimized GFP in expression construct in minimos vector | Peft-3:ceGFP(2xNLS):tbb-2 UTR | pCFJ1137 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(synthetic introns) | tbb-2 3UTR.ape | Request plasmid |
| pCFJ889 | GFP tagged smu-1 gene in minimos vector | Psmu-1:smu-1:gfp:smu-1UTR | pCFJ889 - pEXP(minimos, cbr-unc-119)[Psmu-1 | smu-1| GFP-smu-1 3UTR].ape | Request plasmid |