LOCUS pCFJ1415___pEXP_ 10953 bp ds-DNA circular 17-OCT-2016 DEFINITION . SOURCE . ORGANISM . COMMENT For details, see Froekjaer-Jensen et al. (2016), https://www.ncbi.nlm.nih.gov/pubmed/27374334 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(7101..7118) /label=M13-fwd /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 4241..4261 /label=SV40 /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 6644..6715 /label=egl-13 NLS /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 7..1342 /label=ttTi5605 left recombination arm /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 prim_transcript 1343..3460 /label=cbr-unc-119(+) /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=#cc66ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 6741..7072 /label=tbb-2 3'UTR /ApEinfo_fwdcolor=#ccff66 /ApEinfo_revcolor=#ccff66 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 9387..10046 /label=Amp /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 10144..10826 /label=colE1 /ApEinfo_fwdcolor=#7f7f7f /ApEinfo_revcolor=#7f7f7f /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(7178..8605) /label=ttTi5605 right /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(2331..2387,2486..2597,2641..3018,3070..3176) /label=cbr-unc-119 /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=#8000ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 3589..4210 /label=Peft-3 /ApEinfo_fwdcolor=#66ccff /ApEinfo_revcolor=#cc66ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(4262..4267,4573..4863,5186..5383,5916..6044, 6557..6643) /label=GFP (codon optimized, PATCs) /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa 61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg 121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt 181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta 241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt 301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta 361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa 421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc 481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa 541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg 601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc 661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat 721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca 781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca 841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga 901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata 961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga 1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat 1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca 1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac 1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc 1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat 1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt 1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc 1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct 1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc 1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct 1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat 1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat 1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct 1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg 1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc 1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca 1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct 2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt 2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc 2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa 2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct 2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg 2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa 2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt 2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat 2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact 2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag 2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc 2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga 2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt 2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc 2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg 2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc 3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct 3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt 3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat 3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga 3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg 3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact 3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat 3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac 3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca 3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc acctttggtc 3601 ttttattgtc aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat 3661 aaaataaaga cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga 3721 gaaaaagtat gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg 3781 ctaggagtta gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa 3841 attaacgtga gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg 3901 tttttatctt tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt 3961 caaacattca gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg 4021 ctttcaacaa atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt 4081 ttaaaaatat tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc 4141 actcttctta cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctcactc 4201 cgtagcagcc ATGGCAAGTT TGTACAAAAA AGCAGGCTCG CCAAAGAAGA AGCGTAAGGT 4261 GTCCAAGgta agttttctta tgggaaagaa ggaaaaaacc gagattttac ttgaaaaatt 4321 gaatttttcg cgggattttc accaaaaatt gttgaatatt cattatttca cgctgtaaaa 4381 caaaaaaaaa aaaaatcaaa aactacgttg aaatcgcgtt tttaagcgaa ttttcttcag 4441 aattgccaga ttttaacccc aaattttgca gtttttaaat aaaatttcac cttttcggct 4501 caaattgtag attttcctga aaatttagta caaaaaacaa tttcctcgta aatttttcaa 4561 atagattttc agGGAGAGGA GCTCTTCACC GGAGTCGTCC CAATCCTCGT CGAGCTCGAC 4621 GGAGACGTCA ACGGACACAA GTTCTCCGTC TCCGGAGAGG GAGAGGGAGA CGCCACCTAC 4681 GGAAAGCTCA CCCTCAAGTT CATCTGCACC ACCGGAAAGC TCCCAGTCCC ATGGCCAACC 4741 CTCGTCACCA CCTTCTGCTA CGGAGTCCAA TGCTTCTCCC GTTACCCAGA CCACATGAAG 4801 CGTCACGACT TCTTCAAGTC CGCCATGCCA GAGGGATACG TCCAAGAGCG TACCATCTTC 4861 TTCgtaagtt atgtctacct gcctgcctac cgcctaaatt ttttgtgaag tttcttcaaa 4921 aaatccagaa aaaaaaacaa ttttcatacg attttttccc ttaaaattgt gaattttcat 4981 gctttttagc cccaaaagtc attatttgag aaaaaatttc atacaaaaaa gttttgagaa 5041 atacacaatt ttttaaatgt aattttcaaa ttttcaattt tcaactagaa aattcacaaa 5101 acttgtaaat tttggaccaa aacatttata caattacttt ttttgaatct aataactaca 5161 ataactacaa atttaacatt ttcagAAGGA CGACGGAAAC TACAAGACCC GTGCCGAGGT 5221 CAAGTTCGAG GGAGACACCC TCGTCAACCG TATCGAGCTC AAGGGAATCG ACTTCAAGGA 5281 GGACGGAAAC ATCCTCGGAC ACAAGCTCGA GTACAACTAC AACTCCCACA ACGTCTACAT 5341 CATGGCCGAC AAGCAAAAGA ACGGAATCAA GGTCAACTTC AAGgtaagtt ctttttttga 5401 aaagtgcagt ttgtagtcta attttcattt attttctttt taaaaaacgc atcaattttt 5461 aaatattttg ggacaaaatc cgaaaactgt actaatttgt agtttgtaaa attaaaaaaa 5521 aaacgcaaaa aaatgttttc aaaaatgctt agaataaaaa taaaatttct aaaaaattga 5581 acaaaataaa catttaaaaa ttcaaaaagt ttgaaaaaat gcaaagtttt ttaatgcaaa 5641 aaatatttaa tgtttaaaca aatttaaaaa atgttgtaag aatatgttta gagacagttt 5701 ttaaatgtta aattgcaaaa aaaaaaaagc aaaaaaaaat ttgaaaaatg taacagaaaa 5761 ttttaattaa aaaagtcgtg tttttttcaa acctgcaatt ctttgtaatt ttatttataa 5821 agagaaacaa atcaaattgt atattacgaa aaacgatact aaaattcgaa aatttgcggt 5881 tttttgcgta aaaaatacgg ttccgtaatt ttcagATCCG TCACAACATC GAGGACGGAT 5941 CCGTCCAACT CGCCGACCAC TACCAACAAA ACACCCCAAT CGGAGACGGA CCAGTCCTCC 6001 TCCCAGACAA CCACTACCTC TCCACCCAAT CCGCCCTCTC CAAGgtaagt tcatagattt 6061 tgaaaaaaag tttaagaact gaaaaatgga ataaaaatat ttaagagcat ttttaaatgt 6121 aaaattaaca aaaaaagcgc ctaagaatgt ttccaaaaca gtaaaaaaaa ggtttaaaaa 6181 aatgcaaaaa aaaatttaag atgcttttaa ttacacaaaa aatgaaagta aaaaaaatta 6241 aaatattgaa aaatgtatat ttgtttgaaa aggtacattt ttcaatgcaa aaaatgtttc 6301 taaaaattaa aaaaatatgt ttaaattgtg taaagtgtaa attaaaaaat aaaattatga 6361 aaaagtattg tttcatactt tttctaaatt ttggttgaaa aatttaaaat tccagctttt 6421 tattcacatt atgtgttcta aaaatctttc cccaattttc gggaccgttt tccagtatat 6481 ttccattttt caaaaagatt tttaactgaa attcatgttt tcaatgctaa aaatcaataa 6541 aaaaagaatt tttcagGACC CAAACGAGAA GCGTGACCAC ATGGTCCTCC TCGAGTTCGT 6601 CACCGCCGCC GGAATCACCC ACGGAATGGA CGAGCTCTAC AAGTCCCGTC GTCGTAAGGC 6661 CAACCCAACC AAGCTCTCCG AGAACGCCAA GAAGCTCGCC AAGGAGGTCG AGAACAACCC 6721 AGCTTTCTTG TACAAAGTGG GATAAatgca aaatcctttc aagcattccc ttcttctcta 6781 tcactcttct ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt 6841 attattttat gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat 6901 caaaatgtga ttctgtctga tgtactttca caatctctct tcaattccat tttgaagtgc 6961 tttaaacccg aaaggttgag aaaaatgcga gcgctcaaat atttgtattg tgttcgttga 7021 gtgacccaac aaaaagagga aactttattg tgccgccaag aaaaaagtct cacaacttta 7081 ttatacatag ttgataattc actggccgtc gttttacact cgagacgtac ggtgcgcgcg 7141 atgcattcga agatctgccc actagtgagt cgtattataa gtgcaagtaa gatcagtgtt 7201 tgtttcgata tcagtctgtt tcgtaacggt cttctgtata actacaaaaa agaataaaaa 7261 accgtatcac aactgagaaa aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt 7321 attttgtaaa aaatcgagtt tttctccaaa ataacgcacg caatcaaaaa caagaaaact 7381 aacgtgttct cctgggcgtg gaactcctta tcaagattac cattattgtc tgtcagaatt 7441 gatatttcaa ttatttcata tctagacaaa cccgggtgtc ttgcatttat tagtactgtg 7501 agtttacata caaacgaggc ttactcagat aacatagagt cacctctaat tagcaaaaac 7561 ccatttctaa ttagctattt cgaccagttc taaagtcacc gtaattagaa atggtttttg 7621 tagcttttct aattagaaat gaccccgaga taagtgaaat atggcatttt ctgggttttc 7681 tagtaagaaa tgatcccgca cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa 7741 agtatattac acaattcaag ttacattatt ttcaggaaac aatggcgaag agcaaagcag 7801 agattcaaaa ggaggaaaga gacaacagat atgaacgaag acttcttgaa aagagaaaga 7861 gacatgaggt gagtaaccat gataataaat aatcaagtaa gggctataga tttataaatt 7921 ctggagttaa cagtttcgaa aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata 7981 ggggaatagc ctaaacgagt gagaaaatta aatgtttgtt attttttcaa aaatgtctgc 8041 agggccgtta tcctttcaaa tgcactctat caaacataga tcatatccat catatcttat 8101 ccagaattta tatcatatcc aagattgaga gcgtttcaag gaaaatctca aacaaccata 8161 caattttgta gatcgacaac aaggcctcca cttcttcatc gatggcctct gcagcacctg 8221 caccaccaag tttgtatttt tcatattaat ataaagtgag ccacgcatag tcgtttttag 8281 gatttttgcc agatcgatgt cgcgcaaaca gactgttttt ttttctttga aagccgtgcg 8341 tgacatgggc cacggattgt aacgccgcgc tggatggata aaaatttatt cagtttttta 8401 gcctttacaa aatatggttc gactactttc tttattttta catttttata ggtgctcatt 8461 gagctgagat attttgggta taatcggaaa atcgctgatt ttggtcattt ttctgcacta 8521 aagattacac aaaaagttca gtatttagat tcattgttga aaattgggga tgatctacag 8581 atacatatcg ccgtgttcaa aaacacacgt gacgtcgtga ctgggaaaac cctggcgtta 8641 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 8701 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc 8761 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 8821 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 8881 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 8941 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 9001 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 9061 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 9121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 9181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 9241 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 9301 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 9361 ttcgccccga agaacgtttt ccaatgATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 9421 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 9481 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 9541 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 9601 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 9661 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 9721 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 9781 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 9841 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 9901 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 9961 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 10021 TAGGTGCCTC ACTGATTAAG CATTGGTAAc tgtcagacca agtttactca tatatacttt 10081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 10141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 10201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 10261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 10321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 10381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 10441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 10501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 10561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 10621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 10681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 10741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 10801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggtcttttgc tggccttttg 10861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 10921 agtgagctga taccagcgga taacaatttc aca //