About the miniMos transposon
The miniMos transposon is a method to carry transgenes into the C. elegans genome. The method has the advantages:
- The insertion frequency and fidelity is high
- The exact insertion site can be determined
- Large transgenes can be inserted
- The miniMos element is active in C. elegans, C. briggsae and natural isolates.
- Transgenes are expressed in the germline at high frequency.
The method works by inserting a transgene into a modified Mos1 element (miniMos). The miniMos transposon can carry the inserted transgene (together with a selection marker) into the genome. Insertions are generated directly by injecting worms and take approximately 1 week to generate. The insertion site is random and can be identified by inverse PCR on the strain.
Requirements
- The transgene cloned into the appropriate miniMos vector (different selection markers)
Schematic of method
