LOCUS pCFJ1137___pEXP_ 9486 bp ds-DNA circular 17-OCT-2016 DEFINITION . SOURCE . ORGANISM . COMMENT For details, see Froekjaer-Jensen et al. (2016), https://www.ncbi.nlm.nih.gov/pubmed/27374334 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(5634..5651) /label=M13-fwd /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 4241..4261 /label=SV40 /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 5177..5248 /label=egl-13 NLS /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 7..1342 /label=ttTi5605 left recombination arm /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 prim_transcript 1343..3460 /label=cbr-unc-119(+) /ApEinfo_fwdcolor=#cc66ff /ApEinfo_revcolor=#cc66ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 5274..5605 /label=tbb-2 3'UTR /ApEinfo_fwdcolor=#ccff66 /ApEinfo_revcolor=#ccff66 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 7920..8579 /label=Amp /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 8677..9359 /label=colE1 /ApEinfo_fwdcolor=#7f7f7f /ApEinfo_revcolor=#7f7f7f /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(5711..7138) /label=ttTi5605 right /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(2331..2387,2486..2597,2641..3018,3070..3176) /label=cbr-unc-119 /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=#8000ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(4262..4267,4319..4609,4661..4858,4910..5038, 5090..5176) /label=GFP (codon optimized) /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 3589..4210 /label=Peft-3 /ApEinfo_fwdcolor=#66ccff /ApEinfo_revcolor=#cc66ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa 61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg 121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt 181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta 241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt 301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta 361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa 421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc 481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa 541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg 601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc 661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat 721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca 781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca 841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga 901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata 961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga 1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat 1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca 1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac 1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc 1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat 1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt 1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc 1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct 1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc 1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct 1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat 1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat 1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct 1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg 1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc 1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca 1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca aTaggtttct 2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt 2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc 2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa 2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct 2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg 2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa 2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt 2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat 2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact 2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag 2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc 2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga 2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt 2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc 2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg 2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc 3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct 3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt 3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat 3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga 3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg 3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact 3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat 3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac 3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca 3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc acctttggtc 3601 ttttattgtc aacttccatt ggttcttcca ttgtttctgt taaattaatg aatttttcat 3661 aaaataaaga cattatacaa tataaaaatg aagaatttat tgaaaataaa ctgccagaga 3721 gaaaaagtat gcaacactcc cgccgagagt gtttgaaatg gtgtacggta cattttcgtg 3781 ctaggagtta gatgtgcagg cagcaacgag agggggagag atttttttgg gccttgtgaa 3841 attaacgtga gttttctggt catctgacta atcatgttgg ttttttgttg gtttattttg 3901 tttttatctt tgtttttatc cagattagga aatttaaatt ttatgaattt ataatgaggt 3961 caaacattca gtcccagcgt ttttcctgtt ctcactgttt agtcgaattt ttattttagg 4021 ctttcaacaa atgttctaac tgtcttattt gtgacctcac tttttatatt tttttaattt 4081 ttaaaaatat tagaagtttc taggataatt ttttcgactt ttattctctc taccgtccgc 4141 actcttctta cttttaaatt aaattgtttt tttttcagtt gggaaacact ttgctcactc 4201 cgtagcagcc ATGGCAAGTT TGTACAAAAA AGCAGGCTCG CCAAAGAAGA AGCGTAAGGT 4261 GTCCAAGgta agttttctag agacctttac taactaacta ggtctcatag attttcagGG 4321 AGAGGAGCTC TTCACCGGAG TCGTCCCAAT CCTCGTCGAG CTCGACGGAG ACGTCAACGG 4381 ACACAAGTTC TCCGTCTCCG GAGAGGGAGA GGGAGACGCC ACCTACGGAA AGCTCACCCT 4441 CAAGTTCATC TGCACCACCG GAAAGCTCCC AGTCCCATGG CCAACCCTCG TCACCACCTT 4501 CTGCTACGGA GTCCAATGCT TCTCCCGTTA CCCAGACCAC ATGAAGCGTC ACGACTTCTT 4561 CAAGTCCGCC ATGCCAGAGG GATACGTCCA AGAGCGTACC ATCTTCTTCg taagttatgt 4621 agagaccttt actaactaac taggtctcaa acattttcag AAGGACGACG GAAACTACAA 4681 GACCCGTGCC GAGGTCAAGT TCGAGGGAGA CACCCTCGTC AACCGTATCG AGCTCAAGGG 4741 AATCGACTTC AAGGAGGACG GAAACATCCT CGGACACAAG CTCGAGTACA ACTACAACTC 4801 CCACAACGTC TACATCATGG CCGACAAGCA AAAGAACGGA ATCAAGGTCA ACTTCAAGgt 4861 aagttcttta gagaccttta ctaactaact aggtctcagt aattttcagA TCCGTCACAA 4921 CATCGAGGAC GGATCCGTCC AACTCGCCGA CCACTACCAA CAAAACACCC CAATCGGAGA 4981 CGGACCAGTC CTCCTCCCAG ACAACCACTA CCTCTCCACC CAATCCGCCC TCTCCAAGgt 5041 aagttcataa gagaccttta ctaactaact aggtctcaga atttttcagG ACCCAAACGA 5101 GAAGCGTGAC CACATGGTCC TCCTCGAGTT CGTCACCGCC GCCGGAATCA CCCACGGAAT 5161 GGACGAGCTC TACAAGTCCC GTCGTCGTAA GGCCAACCCA ACCAAGCTCT CCGAGAACGC 5221 CAAGAAGCTC GCCAAGGAGG TCGAGAACAA CCCAGCTTTC TTGTACAAAG TGGGATAAat 5281 gcaaaatcct ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa 5341 ttctctcgct aatttatttg cttttttaat gttattattt tatgactttt tatagtcact 5401 gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt 5461 tcacaatctc tcttcaattc cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg 5521 cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta 5581 ttgtgccgcc aagaaaaaag tctcacaact ttattataca tagttgataa ttcactggcc 5641 gtcgttttac actcgagacg tacggtgcgc gcgatgcatt cgaagatctg cccactagtg 5701 agtcgtatta taagtgcaag taagatcagt gtttgtttcg atatcagtct gtttcgtaac 5761 ggtcttctgt ataactacaa aaaagaataa aaaaccgtat cacaactgag aaaaaagaca 5821 aaacttattg aacagtgttc ctttcaaaaa attattttgt aaaaaatcga gtttttctcc 5881 aaaataacgc acgcaatcaa aaacaagaaa actaacgtgt tctcctgggc gtggaactcc 5941 ttatcaagat taccattatt gtctgtcaga attgatattt caattatttc atatctagac 6001 aaacccgggt gtcttgcatt tattagtact gtgagtttac atacaaacga ggcttactca 6061 gataacatag agtcacctct aattagcaaa aacccatttc taattagcta tttcgaccag 6121 ttctaaagtc accgtaatta gaaatggttt ttgtagcttt tctaattaga aatgaccccg 6181 agataagtga aatatggcat tttctgggtt ttctagtaag aaatgatccc gcacgtagtg 6241 attccgcgtt acaagccaaa ttcaaattaa aaaagtatat tacacaattc aagttacatt 6301 attttcagga aacaatggcg aagagcaaag cagagattca aaaggaggaa agagacaaca 6361 gatatgaacg aagacttctt gaaaagagaa agagacatga ggtgagtaac catgataata 6421 aataatcaag taagggctat agatttataa attctggagt taacagtttc gaaaaaaagt 6481 agttttcata tgtgcgaagg aatatcaaaa ataggggaat agcctaaacg agtgagaaaa 6541 ttaaatgttt gttatttttt caaaaatgtc tgcagggccg ttatcctttc aaatgcactc 6601 tatcaaacat agatcatatc catcatatct tatccagaat ttatatcata tccaagattg 6661 agagcgtttc aaggaaaatc tcaaacaacc atacaatttt gtagatcgac aacaaggcct 6721 ccacttcttc atcgatggcc tctgcagcac ctgcaccacc aagtttgtat ttttcatatt 6781 aatataaagt gagccacgca tagtcgtttt taggattttt gccagatcga tgtcgcgcaa 6841 acagactgtt tttttttctt tgaaagccgt gcgtgacatg ggccacggat tgtaacgccg 6901 cgctggatgg ataaaaattt attcagtttt ttagccttta caaaatatgg ttcgactact 6961 ttctttattt ttacattttt ataggtgctc attgagctga gatattttgg gtataatcgg 7021 aaaatcgctg attttggtca tttttctgca ctaaagatta cacaaaaagt tcagtattta 7081 gattcattgt tgaaaattgg ggatgatcta cagatacata tcgccgtgtt caaaaacaca 7141 cgtgacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 7201 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 7261 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg 7321 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 7381 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 7441 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 7501 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 7561 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 7621 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 7681 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 7741 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 7801 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 7861 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatgA 7921 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 7981 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 8041 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 8101 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 8161 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 8221 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 8281 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 8341 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 8401 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 8461 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 8521 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 8581 AActgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 8641 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 8701 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 8761 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 8821 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 8881 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 8941 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 9001 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 9061 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 9121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 9181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 9241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 9301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 9361 ttttacggtt cctggtcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 9421 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccagc ggataacaat 9481 ttcaca //