LOCUS pCFJ212___pDEST_ 9554 bp ds-DNA circular 03-MAR-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT For description, see Frokjaer-Jensen et al. (2012), Nature Methods, 9:117-8 COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin 1249..1931 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 5755..5879 /label=attR4 /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin complement(763..1214) /label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(7334..7458) /label=attR3 /ApEinfo_fwdcolor=#ff80ff /ApEinfo_revcolor=#ff80ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1..660 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 5919..6224 /label=ccdB /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=#0080ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 2072..2092 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(7466..7483) /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_binding 2044..2066 /label=LacO /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(6569..7225) /label=CmR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS join(4518..4536,4538..5363) /label=CB-unc-119(+) /ApEinfo_fwdcolor=#008000 /ApEinfo_revcolor=#008000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 5364..5647 /label=Cb-unc-119 3'UTR /ApEinfo_fwdcolor=#c5ff29 /ApEinfo_revcolor=#ff7801 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 3530..4517 /label=Cb-unc-119 promoter /ApEinfo_fwdcolor=#3920ff /ApEinfo_revcolor=#f611ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(9015..9032) /label=M13-fwd(1) /ApEinfo_label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 9103..9171 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 2100..3504 /label=Left recomb 10816 /ApEinfo_fwdcolor=#ff2d18 /ApEinfo_revcolor=#2015ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(7575..9008) /label=right recomb 10816 /ApEinfo_fwdcolor=#ff2d18 /ApEinfo_revcolor=#2015ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA 61 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 121 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 181 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 241 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 301 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 361 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 421 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 481 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 541 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 601 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 661 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTACCCCG GTTGATAATC 721 AGAAAAGCCC CAAAAACAGG AAGATTGTAT AAGCAAATAT TTAAATTGTA AACGTTAATA 781 TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCcg 841 aaatcggcaa aatccctTAT AAATCAAAAG AATAGCCCGA GATAGGGTTG AGTGTTGTTC 901 CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA 961 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CAAATCAAGT TTTTTGGGGT 1021 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAg cccccgattt agagcttgAC 1081 GGGGAAAGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC 1141 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC 1201 GCTACAGGGC GCGTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 1261 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 1321 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 1381 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 1441 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC 1501 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 1561 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 1621 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 1681 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 1741 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 1801 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 1861 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 1921 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTAATGTG 1981 AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG 2041 TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGGGTCACCc 2101 aaggcgacaa cgtaagtgac tgaaggcatt aaaggtacat gcgtccgcgc aagtgtccgc 2161 ttttgctggc ctaacaagta agagaaacaa gtaagaaaga gtaagagaaa actcacccga 2221 ggtgtctctt ctagcaaccg cctattcaga taattttaag ccagttcgag agttatttgt 2281 cgcatggtat agaagctcgt tatttacatt aaatttttag tcttcccgca gaagtggacg 2341 atgcgatggg ctacttcaaa aatctgacgc cacgtgtcaa ggaccttcac aagagcatga 2401 caaacttgga agatattagc aagtggcaga agaaggccag tttctctggc acccttgaga 2461 attactcgcg tctcggtgac aagatcaatg tgaaaccatt tatggatgct gttgatgcta 2521 gaatgggtgc cgaagctgat gccgtgaaag gggtataaag ctactgttgc acgacgactc 2581 acagactaat cattccaggt cctcgcgatt tgtgaaaaat acaagtcatt ctaccaaaac 2641 gagggaaaac ttcacgcgga cagtatcgcc aatttgaata ggactcggct cgacatggac 2701 agtgcggcgg ataaatatgc gaacaacgag actgaagtta acaagactcg tttggataac 2761 agtaccacgg aatttgaagt ggcttgtgag aggtttgtag gtctttcgta ggcacgatat 2821 ccttatttga atgtgtttca gaatgcgaga actggcgaac ggaatcaaga caattgaatc 2881 gaaccattct tcctggcaag acggtcttat gaaggagatt aaagtggcgt tgcgtaaata 2941 aagaaaggat cctctttgaa aataaagatt ttctacattc acttattttt ttagctttat 3001 acttttcaga tcgaatgaac tgtttccaac gtttccatat taattttttt tagttaaaaa 3061 agttgggtat taacagtttt gttaacagtt ttgtcttccg acagatgtga gacggaaaaa 3121 tggccaaaat tcgagatttt gactaaaatc agtgtagctt tttcaaaatt ttgaaccggc 3181 atgaaaaagt ttgaaaagtt ttcgagaagt ttcaatccgt tcatttgagc ccatgttggg 3241 tctataagat caaaatcgcc cgaactgtta gtcctccttt aagttacgat tattaacaaa 3301 atgtcgccta agtttagttt cacacttgtc tcacactagt cgatgaactg tgtgactact 3361 gtagtgagta cagtagggcc cgtgtaaaaa tacaaaaacc aagaaaaagt aaaccaaaat 3421 aaaaaacgaa tttattgttc actacaacca aactgcaaag ttcttttctt acccatcagc 3481 tcaatcgtgt acttgcgtgg aaacAAATat ggtagcaaac tcacttcgtc tttgagccaa 3541 tttatccaag tccttgtaaa aagtattcga aaattgttaa cggataaaat gtttattata 3601 atatcaaaaa caatttgtca gttgaccact ttttgatata tttgacagaa accgggatga 3661 attggctcaa agttagggcg cccttctatt acagggtttc tgataacaaa ccggttatta 3721 actcccaaca agggatgatt tcaattcatc atgctcaaat tgacccaaat taagttacat 3781 gacaaattca tcgccctttt caactcttct tggctcatca tctgttattc tgttcctatt 3841 ctgtctgcac cctatacccc tttgcatact ctctcgtcat tcctttcttg gatagtgtct 3901 tcttctcccc agctctgcta cttctatgac ttgcgcccgc tgcttttccg ctcgttcctc 3961 tcctctctga cgtcttcttc tgcttctttt ctccttcctt tttcccttct caatcttcct 4021 ctttttccca tttctgtcaa tcattcgaag aagaagaaga agaagaccct cattcatttc 4081 attttttttt ctgtcggtgt gtgctgctcg gttaagagtg agctctctat tccacgtctt 4141 cttctttttc ttcttcgatt cgaatcaatc actccaccaa aacgcattcg tttttgggat 4201 tcaccccgcg gttcgcaaTa ggtttctttt tcaaattatt agcgttataa atagaaaaat 4261 ggtggagttt caataaaaat gataatttac aaagtgtatt ttgattacat gtactcaaaa 4321 aggttgaaat ttttcatacc agttttccgg aaatccatct gatatcatta tcgtattttt 4381 ctttttaaaa atgttttcaa aaaaaaacaa aatatagctg gttatttggc accctctaat 4441 taccattttc ctgtcacacc acactctttt ccttcttcca cttctttccg ttttcagccg 4501 cttccaacca aaccgatatg aaagccgagc aacaacgatc gattccaccc ggctcggcga 4561 ccttcccgtc gcaggtgaga ctcagaaaac tagagaaacc gctcaactaa ctcttgatat 4621 ccgatttcat tcttttctct tttctttttg ttgaactttt ccatatttcc agatgccacg 4681 gccaccacca agcaccgaac aaggaatcac aacggaatcg gagcttgcga agaaagctca 4741 aatcactccg aacgacgttt tagcacttcc gggaatcact caaggtatct tgctcccttt 4801 ggatttcagt ttaaaacata aatttaggat tcttatgctc cccatctgcg aacatctata 4861 acatcgagtt caccaagttc caaatccgtg atctggacac tgagcaagtg cttttcgaga 4921 tcgccaaacc ggagaacgat caggagaatg atgagtcgcc acaggagtcg gcaagatacg 4981 tgcgttatag atttgctcca aactttttga aactcaaaac ggtcggagca actgtggaat 5041 tcaaagtagg agacatccca atccatcatt tccgaatgat cgaacgtcac ttcttcaaag 5101 atcgccttct gaagtgtttt gactttgaat tcggattctg tattccgaat tcacgaaaca 5161 actgtgaaca tatctatgag ttccctcaac tctctcaaca actcagtgag tcattattct 5221 aaaaagtaca attcaaaaga ctaatctctt tttcagtgga cgacatgatc aacaatccaa 5281 acgagactcg ttctgacagc ttctatttcg tcgataacaa actcgtcatg cacaacaaag 5341 ccgactactc atatgatgca taaatattta atacaaaaat gttctggata attattctgt 5401 cgaatagaaa aaaaactcca aatgtgatta aattccaata attcctgtct agtttgttcc 5461 ttccccttcc ccttctcatg ttcaatgcat tcctaagctt ttcagttccc cccttgtttc 5521 tatatttttt cgctgtcctg tcacactcgc taaaaacact aatcacacgg aaatctgttt 5581 tcaataaaaa ctccaacttt aactcatttt caatttcaac tgaaagattt tttcattaga 5641 gaatgtctag aactagGCCC GGGCTACGTA ATACGACTCA CTTAAGGCCT TGACTAGAGG 5701 GTACCAGAGC TCACCTAGGc aggaacagct atgaccatga ttacgccaag ctatcaactt 5761 tgtatagaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga 5821 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggt 5881 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg 5941 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata 6001 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata 6061 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg 6121 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga 6181 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt 6241 ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg 6301 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct 6361 taccgaaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca 6421 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca 6481 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg 6541 cttttagtaa gccggatcct ctagattacg ccccgccctg ccactcatcg cagtactgtt 6601 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa 6661 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg 6721 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg 6781 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt 6841 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt 6901 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt 6961 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag 7021 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct 7081 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag 7141 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg 7201 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat 7261 cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag cctgggggtg 7321 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 7381 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa 7441 ctttattata catagttgat aattcactgg ccgtcgtttt acaCTCGAGA CGTACGGTGC 7501 GCGCGATGCA TTCGAAGATC TGCCCACTAG TGAGTCGTAT TAtaagtgca agtaagatca 7561 gtgtttgtAT TTtactatac tttttaaaga cacacggcca aatttcttat tttcctgcta 7621 ttttctccaa atatgattaa aatttgttca tttcaaaatt gcctccaact tgttttgggg 7681 caaaaatttc acaaaacttc acagcaaaat gtgaaacaat attgctatct attgaaaaca 7741 ttaatgatag tcgagtgact tcttgaacca atactcatcg catcaaatgc cttttctcga 7801 atactctgta ataattggca tataaaaaca tacatactca tcagtttgaa agctaacctt 7861 taccataatc attactaaaa ttggatttaa agccggacac caagaaaaaa gatacggtag 7921 ataaactgac agatgagaag tctcgatacg aaataatggc attagaaaag acacatagaa 7981 tggaatgtgg atagtgagaa atggtagaat gaactgaaaa attgagaatt taaggagaaa 8041 tgttattttt aagctgcttt gcttgaaact ttgaatttaa aattttagaa caattactgt 8101 ttcaaaatat ttttattttt atttactcca tttgaaaaaa aagcaacatt gcttgttcaa 8161 tttctgttat tgccttacct gaaccataag taccatattc aattgttgtt gagacttcat 8221 cgaatttgta ctccgacttc ccttatgaat ttcgtatctg ataattgtcc aacaaaccat 8281 cgcaattact gtagttatca ctagaacagc cattatataa atcgtacatg cccatgaaat 8341 tgggtcagca acaaagtcat tcaacgtgta atcatatctt tctagtgatg caattaaata 8401 tgcagatttt ccgattccat tcagattgta tacgatatat tctgatagtt gatcgtcagc 8461 agcatctggg acatagccaa aggtgattag aactgtggat attagagaga caataaatga 8521 tatgattgtg gttgtaagga ccacgttttt ttgtgttctc gttgttctga aagtataatt 8581 tgtagattct taaactcaga atttatcata tttgaactcc gctgtttttg aattaagtaa 8641 tagctgaaac agtgaacatt agtttaaaaa tataatttta caggcacgca caaacttcgg 8701 tgtgggtacg tattacctgc ctcctattga atattcattg tttaaacgtt ttcagaaaaa 8761 aagtgtacat gttttttgga gtatgatgat gatgtagtcg caaggcagat atgaaatgta 8821 ggtaacacag tgcacagatg cgttttttgc caacatcaag ggttgtaagt aacttttcag 8881 agaaaaacat atagaaattt tgtttcaagt atcgtatcag tttttttctg attaacttat 8941 aaaaagaact cttcgacaat ttgaatgctt aatttaaaaa aattaagtcg aatatttcag 9001 catgccaaTG ATCAactggc cgtcgtttta caACGTCGTG ACTGGGAAAA CCCTGGCGTT 9061 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 9121 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCTTCGCT 9181 TGGTAATAAA GCCCGCTTCG GCGGGCTTTT TTTTGTTAAC TACGTCAGGT GGCACTTTTC 9241 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 9301 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 9361 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 9421 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 9481 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 9541 AACGTTCTCC AATG //