LOCUS pMA122___Phsp16_ 3854 bp ds-DNA circular 03-MAR-2013 COMMENT Plasmid made by Michael Ailion. COMMENT For description, see Frøkjær-Jensen et al. (2012), Nature Methods, 9:117-8 COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers primer_bind complement(1438..1455) /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1526..1594 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 3834..3854 /label=attB4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(1410..1430) /label=attB3 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(1057..1077) /label=attB2 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 502..522 /label=attB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 3798..3818 /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 2988..3670 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2231..2890 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 1078..1409 /label=tbb-2 3' UTR /ApEinfo_fwdcolor=#8000ff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 528..1049 /label=peel-1 cDNA no stop /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 1..501 /label=hsp16-41 /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ctgcaggtcg actctagagg atcaccaaaa acggaacgtt gagctggacg gaaatagtgg 61 taaagtgaca tgattatagt ttgaagattt ctaatttcac aattagagca aatgttgttc 121 ggtatttatt ttcaacggta tttatactat tttccacctt tttctagaac attcgagctg 181 cttgttgcaa aaggagggcg actcacattc ggtacatgga aaagtagtgt acacaataaa 241 gagacccaga tacattttcc gtctgcgtct ctttgcaccc accgggagta ttttcaaacg 301 aatgcatcta ggaccttcta gaacattctg taaggctgca gaatgcgggt atataaggaa 361 agcgggctca gaggaagcca acacgctttg ttctagtgca tctaaaaaac ttcgaaaatc 421 ctcatcggga tcctatcgat tcgcggccgc tgtacacccg ggtgccctat agtgagtcgt 481 attggtaccg gtagaaaaaa tCAAGTTTGT ACAAAAAAGC AGGCTTCATG CGCTTTGATT 541 TCCAAAACTT AAAATTTTCG ATGATTTTCA TATTTTTGTG GAACATCATA GTCGGATTTT 601 TACTTGCTCT TGTAAAGATT TTCAAAATCT ACATGTATCT TGATCTGCCT GAAAATGTAT 661 GGGTAAATCG GTTTGCGCAT GTTATTGCTC TTATTGGATC AATTGCTTCA TTGTGGTTAA 721 TTTTTTTTAG CCCATTTGAA ATTAAGCAAG GAAGATTTTC TGTCTCGTCA AAAACTATCC 781 TGCTTCTTGT TTGCAAGGGT TTGATTGGCT TGATATTTCT TGCACAGATT TTCGCCCATG 841 TCCTTTACAT TGACCCATGC CAGAATATGC TCTTATCATT GAACTCTGCA CTGTTCTCCT 901 TGCTGCTGAA TTATTATGAG TCTCGTGGTG CGATCCATCC GTTGATGGAA TTAGTTCAAA 961 CAGCTGGCCT GAGACCAACT TTGTGCGCTG CTGTACGATT TAATTGTCTC AACTCAACTG 1021 CTCATATTGA TCCAAGTGTT GAAAATCCAT GAGACCCAGC TTTCTTGTAC AAAGTGGgat 1081 aaatgcaaaa tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa 1141 aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt 1201 cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt 1261 actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa 1321 aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac 1381 tttattgtgc cgccaagaaa aaagtctcaC AACTTTATTA TACATAGTTG ATAATTCACT 1441 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT 1501 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC 1561 TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG ATGCGGTATT TTCTCCTTAC 1621 GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC 1681 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG 1741 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA 1801 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT 1861 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG 1921 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT 1981 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT 2041 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC 2101 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG 2161 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG 2221 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA 2281 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA 2341 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC 2401 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC 2461 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG 2521 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC 2581 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA 2641 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT 2701 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT 2761 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG 2821 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT 2881 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT 2941 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT 3001 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3061 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3121 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3181 CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3241 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3301 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3361 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3421 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 3481 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3541 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3601 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3661 CAACGCGGCC TTTTTACGGT TCCTGGTCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC 3721 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCAG 3781 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TATCAACTTT 3841 GTATAGAAAA GTTG //