LOCUS pCFJ1663___pEXPm 7192 bp ds-DNA circular 22-JAN-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Generated by two fragment gateway reaction. COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers promoter 3298..3797 /label=hsp16-41 /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 58..163 /label=MCS /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 4323..4654 /label=tbb-2 3' utr /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#00ffff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6964..7185 /label=Mos1 drosophila /ApEinfo_fwdcolor=#7b1bff /ApEinfo_revcolor=#7b1bff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 6936..6963 /label=Artificial 3'ITR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3798..4319 /label=peel-1 cDNA /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2914..3210 /label=Mos1 drosophila(1) /ApEinfo_label=Mos1 drosophila /ApEinfo_fwdcolor=#7b1bff /ApEinfo_revcolor=#7b1bff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 6120..6802 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4325..4327 /label=Stop /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 4658..4726 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 5363..6022 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 218..968 /label=Prps-0 /ApEinfo_fwdcolor=#ff101a /ApEinfo_revcolor=#ff101a /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 37..57 /label=attB4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(164..184) /label=attB3 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 969..2096 /label=hygR CeOpt /ApEinfo_fwdcolor=#1227ff /ApEinfo_revcolor=#1227ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 variation 4320..4322 /label=Stop(1) /ApEinfo_label=Stop /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 1146..1196 /label=SynIVS.R /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(192..208) /label=M13F(-20) /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 2040..2090 /label=SynIVS.S /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 6..21 /label=M13R(-24) /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 2097..2898 /label=unc-54 3'UTR /ApEinfo_fwdcolor=#ff0118 /ApEinfo_revcolor=#ff0118 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 2173..2223 /label=SynIVS.L /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgAGC 61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGATA 121 TCAAGCTTAT CGATACCGTC GACCTCGAGG GGGGGCCCGG TACcaacttt attatacata 181 gttgataatt cactggccgt cgttttacag gccggatATG AAGATGAACG CAAGGAGCCT 241 GATGCAGCGA CTCTTGCTAG GAGATCATCA GAAAGCTTAT ATTTGATTAG TGACGTCGCA 301 TCAAAGCTGA ACACATAAAT TCTTTAATAT TTTATATCCC CATATTATGC ACCTGAGAGG 361 AATGAAGAAA TTTGCTGATA CTCCCGGTGA ATTCATGTCT TGATGGTTGT GCGCATTGTG 421 GTAATATCTG AAAAATTTCG AAGTCAGTTT TCTGATAAGG CAACAACTAA TAAATTGAGA 481 ATCAATTTAC CCTTAAGGAA CGATGTTCGC TTCTGAATTT ATTAATAAAT TTTTCCGTAA 541 TTCAATTCAA AATTGTCATA TTTAAGAAAA AAACATCAAA AATGACAAAT TCAATATTTT 601 AATTAATTTT TGCTTTCGTC GTAAATCTAC ACACGCGTCT CTTCCGTGCG AGAGTCCAAG 661 CCAGCAGCCA AATTCGTTGA CTGAGTATTC AACGTTTATA CGTTGTCGGC AACGAGAAAT 721 AGGAAAATGC ATCGGGAAAT GTTCTTTTTT CGATTTTTTC CAAGGTTTTG ACAAATTTTA 781 CCACGAATTT TGCTATGTTT TCAATTAAAA AATATGTTAT TCAACTGTTT CTATGAGGAA 841 AATAAGGCTT TGCATGTAAT TTTCTTATTC AGCATAATTT TTAATTAATT TGAATTTTCT 901 GTCCTAACGT TTATTTTGTT TTCTTGGTTA TGACTGATCT GAAATTAATT TTTGAATTTT 961 AAGGTAATAT GAAAAAACCC GAGTTGACCG CCACATCCGT AGAGAAGTTC CTCATCGAGA 1021 AGTTCGACTC CGTCTCCGAC CTCATGCAAC TCTCCGAGGG AGAGGAGTCC CGTGCCTTCT 1081 CCTTCGACGT CGGAGGACGT GGATACGTCC TCCGTGTCAA CTCCTGCGCC GACGGATTCT 1141 ACAAGGTAAG TTTAAACATA TATATACTAA CTAACCCTGA TTATTTAAAT TTTCAGGACC 1201 GTTACGTCTA CCGTCACTTC GCCTCCGCCG CCCTCCCAAT CCCAGAGGTC CTCGACATCG 1261 GAGAGTTCTC CGAGTCCCTC ACCTACTGCA TCTCCCGTCG TGCCCAAGGA GTCACCCTCC 1321 AAGACCTCCC AGAGACCGAG CTCCCAGCCG TCCTCCAACC AGTCGCCGAG GCCATGGACG 1381 CCATCGCCGC CGCCGACCTC TCCCAAACCT CCGGATTCGG ACCATTCGGA CCACAAGGAA 1441 TCGGACAATA CACCACCTGG CGTGACTTCA TCTGCGCCAT CGCCGACCCA CACGTCTACC 1501 ACTGGCAAAC CGTCATGGAC GACACCGTCT CCGCCTCCGT CGCCCAAGCC CTCGACGAGC 1561 TCATGCTCTG GGCCGAGGAC TGCCCAGAGG TCCGTCACCT CGTCCACGCC GACTTCGGAT 1621 CCAACAACGT CCTCACCGAC AACGGACGTA TCACCGCCGT CATCGACTGG TCCGAGGCCA 1681 TGTTCGGAGA CTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGCGTCCA TGGCTCGCCT 1741 GCATGGAGCA ACAAACCCGT TACTTCGAGC GTCGTCACCC AGAGCTCGCC GGATCCCCAC 1801 GTCTCCGTGC CTACATGCTC CGTATCGGAC TCGACCAACT CTACCAATCC CTCGTCGACG 1861 GAAACTTCGA CGACGCCGCC TGGGCCCAAG GACGTTGCGA CGCCATCGTC CGTTCCGGAG 1921 CCGGAACCGT CGGACGTACC CAAATCGCCC GTCGTTCCGC CGCCGTCTGG ACCGACGGAT 1981 GCGTCGAGGT CCTCGCCGAC TCCGGAAACC GTCGTCCATC CACCCGTCCA CGTGCCAAGG 2041 TAAGTTTAAA CAGTTCGGTA CTAACTAACC ATACATATTT AAATTTTCAG GAGTAAAATT 2101 CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC 2161 GCTGTCATCA GAGTAAGTTT AAACTGAGTT CTACTAACTA ACGAGTAATA TTTAAATTTT 2221 CAGCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT 2281 GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT 2341 TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA 2401 ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT 2461 AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA 2521 TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG 2581 TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA 2641 TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT 2701 TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT 2761 CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT 2821 CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG 2881 TGGAGTAGTG TCTATGGGAG CTTGATccgg atcTGCGGCT TACTCACCAG ACCTGGCCCC 2941 ATCCGATTAC CACCTATTCG CTTCGATGGG ACACGCACTC GCTGAGCAGC GCTTCGATTC 3001 TTACGAAAGT GTGAAAAAAT GGCTCGATGA ATGGTTCGCC GCAAAAGACG ATGAGTTCTA 3061 CTGGCGTGGA ATCCACAAAT TGCCCGAGAG ATGGGAAAAA TGTGTAGCTA GCGACGGCAA 3121 ATACTTTGAA TAAATGATTT TTTCTTTTTC CACAAAATTT AACGTGTTTT TTGATTTAAA 3181 AAAAACGACA TTTCATACTT GTACACCTGA TATCTAGATA CCTAGGTGAG CTCTGGTACC 3241 Cccctggcgt tacccaactt aatcgccttg cagcaGAGTC GTATTACGTA GCCCGGCctg 3301 caggtcgact ctagaggatc accaaaaacg gaacgttgag ctggacggaa atagtggtaa 3361 agtgacatga ttatagtttg aagatttcta atttcacaat tagagcaaat gttgttcggt 3421 atttattttc aacggtattt atactatttt ccaccttttt ctagaacatt cgagctgctt 3481 gttgcaaaag gagggcgact cacattcggt acatggaaaa gtagtgtaca caataaagag 3541 acccagatac attttccgtc tgcgtctctt tgcacccacc gggagtattt tcaaacgaat 3601 gcatctagga ccttctagaa cattctgtaa ggctgcagaa tgcgggtata taaggaaagc 3661 gggctcagag gaagccaaca cgctttgttc tagtgcatct aaaaaacttc gaaaatcctc 3721 atcggatcct atcgattcgc ggccgctgta cacccgggtg ccctatagtg agtcgtattg 3781 gtaccggtag aaaaaatATG CGCTTTGATT TCCAAAACTT AAAATTTTCG ATGATTTTCA 3841 TATTTTTGTG GAACATCATA GTCGGATTTT TACTTGCTCT TGTAAAGATT TTCAAAATCT 3901 ACATGTATCT TGATCTGCCT GAAAATGTAT GGGTAAATCG GTTTGCGCAT GTTATTGCTC 3961 TTATTGGATC AATTGCTTCA TTGTGGTTAA TTTTTTTTAG CCCATTTGAA ATTAAGCAAG 4021 GAAGATTTTC TGTCTCGTCA AAAACTATCC TGCTTCTTGT TTGCAAGGGT TTGATTGGCT 4081 TGATATTTCT TGCACAGATT TTCGCCCATG TCCTTTACAT TGACCCATGC CAGAATATGC 4141 TCTTATCATT GAACTCTGCA CTGTTCTCCT TGCTGCTGAA TTATTATGAG TCTCGTGGTG 4201 CGATCCATCC GTTGATGGAA TTAGTTCAAA CAGCTGGCCT GAGACCAACT TTGTGCGCTG 4261 CTGTACGATT TAATTGTCTC AACTCAACTG CTCATATTGA TCCAAGTGTT GAAAATCCAt 4321 gagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt 4381 gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt 4441 atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct 4501 gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg 4561 agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag 4621 gaaactttat tgtgccgcca agaaaaaagt ctcaagctgg cgtaatagcg aagaggcccg 4681 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 4741 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 4801 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 4861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 4921 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 4981 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 5041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 5101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 5161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 5221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 5281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 5341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 5401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 5461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 5521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 5581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 5641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 5701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 5761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 5821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 5881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 5941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 6001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 6061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 6121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 6181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 6241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 6301 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 6361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 6421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 6481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 6541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 6601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 6661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 6721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 6781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggtc ttttgctggc cttttgctca 6841 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 6901 agctgatacc GCACCGTACG TCTCGAGGAA TTCTATCAGG TGTACAAGTA TGAAATGTCG 6961 TTTCGAACAT ATAGATGTCT CGCAAACGTA AATATTTATC GATTGTCATA AAACTTTGAC 7021 CTTGTGAAGT GTCAACCTTG ACTGTCGAAC CACCATAGTT TGGCGCGAAT TGAGCGTCAT 7081 AATTGTTTAC TCTCAGTGCA GTCAACATGT CGAGTTTCGT GCCGAATAAA GAGCAAACGC 7141 GGACAGTATT AATTTTCTGT TTTCATTTGA AGAAAACAGC TGCGGccgga tc //