LOCUS pCFJ1662___pEXPm 5828 bp ds-DNA circular 22-JAN-2014 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Generated by two fragment Gateway reaction. COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers misc_feature 58..163 /label=MCS /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5600..5821 /label=Mos1 drosophila /ApEinfo_fwdcolor=#7b1bff /ApEinfo_revcolor=#7b1bff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5572..5599 /label=Artificial 3'ITR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2914..3210 /label=Mos1 drosophila(1) /ApEinfo_label=Mos1 drosophila /ApEinfo_fwdcolor=#7b1bff /ApEinfo_revcolor=#7b1bff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 4756..5438 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3294..3362 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3999..4658 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(192..208) /label=M13F(-20) /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 6..21 /label=M13R(-24) /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 218..968 /label=Prps-0 /ApEinfo_fwdcolor=#ff101a /ApEinfo_revcolor=#ff101a /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 2040..2090 /label=SynIVS.S /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 969..2096 /label=hygR CeOpt /ApEinfo_fwdcolor=#1227ff /ApEinfo_revcolor=#1227ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 2097..2898 /label=unc-54 3'UTR /ApEinfo_fwdcolor=#ff0118 /ApEinfo_revcolor=#ff0118 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 1146..1196 /label=SynIVS.R /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 2173..2223 /label=SynIVS.L /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 37..57 /label=attB4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(164..184) /label=attB3 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgAGC 61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCCC CGGGCTGCAG GAATTCGATA 121 TCAAGCTTAT CGATACCGTC GACCTCGAGG GGGGGCCCGG TACcaacttt attatacata 181 gttgataatt cactggccgt cgttttacag gccggatATG AAGATGAACG CAAGGAGCCT 241 GATGCAGCGA CTCTTGCTAG GAGATCATCA GAAAGCTTAT ATTTGATTAG TGACGTCGCA 301 TCAAAGCTGA ACACATAAAT TCTTTAATAT TTTATATCCC CATATTATGC ACCTGAGAGG 361 AATGAAGAAA TTTGCTGATA CTCCCGGTGA ATTCATGTCT TGATGGTTGT GCGCATTGTG 421 GTAATATCTG AAAAATTTCG AAGTCAGTTT TCTGATAAGG CAACAACTAA TAAATTGAGA 481 ATCAATTTAC CCTTAAGGAA CGATGTTCGC TTCTGAATTT ATTAATAAAT TTTTCCGTAA 541 TTCAATTCAA AATTGTCATA TTTAAGAAAA AAACATCAAA AATGACAAAT TCAATATTTT 601 AATTAATTTT TGCTTTCGTC GTAAATCTAC ACACGCGTCT CTTCCGTGCG AGAGTCCAAG 661 CCAGCAGCCA AATTCGTTGA CTGAGTATTC AACGTTTATA CGTTGTCGGC AACGAGAAAT 721 AGGAAAATGC ATCGGGAAAT GTTCTTTTTT CGATTTTTTC CAAGGTTTTG ACAAATTTTA 781 CCACGAATTT TGCTATGTTT TCAATTAAAA AATATGTTAT TCAACTGTTT CTATGAGGAA 841 AATAAGGCTT TGCATGTAAT TTTCTTATTC AGCATAATTT TTAATTAATT TGAATTTTCT 901 GTCCTAACGT TTATTTTGTT TTCTTGGTTA TGACTGATCT GAAATTAATT TTTGAATTTT 961 AAGGTAATAT GAAAAAACCC GAGTTGACCG CCACATCCGT AGAGAAGTTC CTCATCGAGA 1021 AGTTCGACTC CGTCTCCGAC CTCATGCAAC TCTCCGAGGG AGAGGAGTCC CGTGCCTTCT 1081 CCTTCGACGT CGGAGGACGT GGATACGTCC TCCGTGTCAA CTCCTGCGCC GACGGATTCT 1141 ACAAGGTAAG TTTAAACATA TATATACTAA CTAACCCTGA TTATTTAAAT TTTCAGGACC 1201 GTTACGTCTA CCGTCACTTC GCCTCCGCCG CCCTCCCAAT CCCAGAGGTC CTCGACATCG 1261 GAGAGTTCTC CGAGTCCCTC ACCTACTGCA TCTCCCGTCG TGCCCAAGGA GTCACCCTCC 1321 AAGACCTCCC AGAGACCGAG CTCCCAGCCG TCCTCCAACC AGTCGCCGAG GCCATGGACG 1381 CCATCGCCGC CGCCGACCTC TCCCAAACCT CCGGATTCGG ACCATTCGGA CCACAAGGAA 1441 TCGGACAATA CACCACCTGG CGTGACTTCA TCTGCGCCAT CGCCGACCCA CACGTCTACC 1501 ACTGGCAAAC CGTCATGGAC GACACCGTCT CCGCCTCCGT CGCCCAAGCC CTCGACGAGC 1561 TCATGCTCTG GGCCGAGGAC TGCCCAGAGG TCCGTCACCT CGTCCACGCC GACTTCGGAT 1621 CCAACAACGT CCTCACCGAC AACGGACGTA TCACCGCCGT CATCGACTGG TCCGAGGCCA 1681 TGTTCGGAGA CTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGCGTCCA TGGCTCGCCT 1741 GCATGGAGCA ACAAACCCGT TACTTCGAGC GTCGTCACCC AGAGCTCGCC GGATCCCCAC 1801 GTCTCCGTGC CTACATGCTC CGTATCGGAC TCGACCAACT CTACCAATCC CTCGTCGACG 1861 GAAACTTCGA CGACGCCGCC TGGGCCCAAG GACGTTGCGA CGCCATCGTC CGTTCCGGAG 1921 CCGGAACCGT CGGACGTACC CAAATCGCCC GTCGTTCCGC CGCCGTCTGG ACCGACGGAT 1981 GCGTCGAGGT CCTCGCCGAC TCCGGAAACC GTCGTCCATC CACCCGTCCA CGTGCCAAGG 2041 TAAGTTTAAA CAGTTCGGTA CTAACTAACC ATACATATTT AAATTTTCAG GAGTAAAATT 2101 CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC 2161 GCTGTCATCA GAGTAAGTTT AAACTGAGTT CTACTAACTA ACGAGTAATA TTTAAATTTT 2221 CAGCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT 2281 GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT 2341 TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA 2401 ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT 2461 AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA 2521 TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG 2581 TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA 2641 TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT 2701 TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT 2761 CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT 2821 CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG 2881 TGGAGTAGTG TCTATGGGAG CTTGATccgg atcTGCGGCT TACTCACCAG ACCTGGCCCC 2941 ATCCGATTAC CACCTATTCG CTTCGATGGG ACACGCACTC GCTGAGCAGC GCTTCGATTC 3001 TTACGAAAGT GTGAAAAAAT GGCTCGATGA ATGGTTCGCC GCAAAAGACG ATGAGTTCTA 3061 CTGGCGTGGA ATCCACAAAT TGCCCGAGAG ATGGGAAAAA TGTGTAGCTA GCGACGGCAA 3121 ATACTTTGAA TAAATGATTT TTTCTTTTTC CACAAAATTT AACGTGTTTT TTGATTTAAA 3181 AAAAACGACA TTTCATACTT GTACACCTGA TATCTAGATA CCTAGGTGAG CTCTGGTACC 3241 Cccctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 3301 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 3361 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 3421 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 3601 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 3661 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 3721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 3781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 3841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 3901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 3961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 4021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 4081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 4141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 4201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 4261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 4321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 4381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 4441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 4501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 4561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 4621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 4681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 4741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 4801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 4861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 4921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 4981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 5041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 5101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 5161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 5221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 5281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 5341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 5401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggtctttt 5461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 5521 ttaccgcctt tgagtgagct gataccGCAC CGTACGTCTC GAGGAATTCT ATCAGGTGTA 5581 CAAGTATGAA ATGTCGTTTC GAACATATAG ATGTCTCGCA AACGTAAATA TTTATCGATT 5641 GTCATAAAAC TTTGACCTTG TGAAGTGTCA ACCTTGACTG TCGAACCACC ATAGTTTGGC 5701 GCGAATTGAG CGTCATAATT GTTTACTCTC AGTGCAGTCA ACATGTCGAG TTTCGTGCCG 5761 AATAAAGAGC AAACGCGGAC AGTATTAATT TTCTGTTTTC ATTTGAAGAA AACAGCTGCG 5821 Gccggatc //