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Verification of insertions into universal mosSCI sites

Posted on by Christian Frøkjær-Jensen — 3 Comments ↓

I’ve had a few people ask me for sequences surrounding the universal mosSCI sites and oligos that can be used to verify insertions and homozygosity of the inserts. I have posted ApE files with the local genomic environment and the oligos we use to verify the position and homozygosity of inserts here.

Posted in Universal MosSCI sites
3 comments on “Verification of insertions into universal mosSCI sites”
  1. Dhaval says:
    January 7, 2014 at 7:40 am

    Hi Christian,

    Thanks for posting the annotated ApE files for these sites. Looks like the OxTi185 file is missing the primer sequences.

    Cheers

    Dhaval

    Reply
    • Christian Frøkjær-Jensen says:
      January 31, 2014 at 10:35 pm

      Hi Dhaval,
      Sorry for the slow reply – I’ve been traveling. When I download the oxTi185 file it does contain the oligos. Will you let me know if you still have trouble with it and I’ll send it to you directly. Cheers, Christian

      Reply
      • Dhaval says:
        February 1, 2014 at 4:18 am

        Hi Christian,

        I downloaded the file again and everything was there. I must have messed up the file the first time round.

        Sorry

        Dhaval

        Reply

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