Detailed protocols
Single-copy insertion
Constitutive Cas9 (plasmid-based)
Heat shock Cas9 (plasmid-based)
Multiplexed w heat shock Cas9 (plasmid-based)
Extra-chromosomal array integration
Two-step Cas9 (protein + sgRNA).
One-step constitutive Cas9 (plasmid).
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Reagents
Strains
| Site | Selection | Strain | Annotated genomic region | CGC |
| Chr. I (7.45 Mb) | unc-119 | CFJ77 | File (ApE) | Strain request |
| Pmlc-2::gfp | CFJ184 | File (ApE) | Strain request | |
| Chr. II (8.24 Mb) | unc-119 | CFJ42 | File (ApE) | Strain request |
| Pmlc-2::gfp | CFJ108 | File (ApE) | Strain request | |
| HygroR | CFJ111 | File (ApE) | Strain request | |
| Chr. III (8.62 Mb) | unc-119 | CFJ94 | File (ApE) | Strain request |