Detailed protocols

Single-copy insertion

Constitutive Cas9 (plasmid-based)

Heat shock Cas9 (plasmid-based)

Multiplexed w heat shock Cas9 (plasmid-based)

Extra-chromosomal array integration

Two-step Cas9 (protein + sgRNA) Coming soon.

Two-step heat shock Cas9 (plasmid) Coming soon.

One-step constitutive Cas9 (plasmid) Coming soon.

Reagents

Strains

SiteSelectionStrainAnnotated genomic regionCGC
Chr. I (7.45 Mb)unc-119CFJ77File (ApE)Strain request
Pmlc-2::gfpCFJ184File (ApE)Strain request
Chr. II (8.24 Mb)unc-119CFJ42File (ApE)Strain request
Pmlc-2::gfpCFJ108File (ApE)Strain request
HygroRCFJ111File (ApE)Strain request
Chr. III (8.62 Mb)unc-119CFJ94File (ApE)Strain request