LOCUS       2__mosTI_chrIV_l        4348 bp    DNA        linear       24-JUN-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >IV:8617211-8619131
COMMENT     MosTI landing site kstSI37 on Chr. IV with empty pSEM246 insertion.
            The exact configuration of your transgene will depend on how you
            cloned it into pSEM246. 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(join(3406..3582,3992..4094,4147..4274))
                     /locus_tag="F41A9.3"
                     /label="F41A9.3"
                     /ApEinfo_label="F41A9.3"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#72fa78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             1943..1999
                     /locus_tag="cbr-unc-119 exons 3/4"
                     /label="cbr-unc-119 exons 3/4"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(353..514,561..672,724..809)
                     /locus_tag="F42A9.8"
                     /label="F42A9.8"
                     /ApEinfo_label="F42A9.8"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#72fa78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             2253..2330
                     /locus_tag="cbr-unc-119 exons 3/4(1)"
                     /label="cbr-unc-119 exons 3/4(1)"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           810..881
                     /locus_tag="3'UTR (wormbase annotation)"
                     /label="3'UTR (wormbase annotation)"
                     /ApEinfo_label="3'UTR (wormbase annotation)"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     3'UTR           complement(3369..3405)
                     /locus_tag="3' UTR Wormbase annotation"
                     /label="3' UTR Wormbase annotation"
                     /ApEinfo_label="3' UTR Wormbase annotation"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             2098..2209
                     /locus_tag="cbr-unc-119 exons 3/4(2)"
                     /label="cbr-unc-119 exons 3/4(2)"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    882..898
                     /locus_tag="LH ChrIII"
                     /label="LH ChrIII"
                     /ApEinfo_label="LH ChrIII"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           882..887
                     /locus_tag="3'UTR"
                     /label="3'UTR"
                     /ApEinfo_label="3'UTR"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    882..898
                     /locus_tag="sgRNA"
                     /label="sgRNA"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     909..942
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     943..946
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        943..946
                     /locus_tag="Punc-119"
                     /label="Punc-119"
                     /ApEinfo_label="Punc-119"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    955..2330
                     /locus_tag="partial cbr-unc-119(+)"
                     /label="partial cbr-unc-119(+)"
                     /ApEinfo_label="partial cbr-unc-119(+)"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3325..3327
                     /locus_tag="sgRNA(1)"
                     /label="sgRNA(1)"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3325..3368
                     /locus_tag="RH chrIII"
                     /label="RH chrIII"
                     /ApEinfo_label="RH chrIII"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2331..2630
                     /locus_tag="cbr-unc-119 (Exons 3-4)"
                     /label="cbr-unc-119 (Exons 3-4)"
                     /ApEinfo_label="cbr-unc-119 (Exons 3-4)"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2682..2788
                     /locus_tag="cbr-unc-119 (Exons 3-4)(1)"
                     /label="cbr-unc-119 (Exons 3-4)(1)"
                     /ApEinfo_label="cbr-unc-119 (Exons 3-4)"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           2789..2901
                     /locus_tag="eft-3 3'UTR"
                     /label="eft-3 3'UTR"
                     /ApEinfo_label="eft-3 3'UTR"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     2902..2935
                     /locus_tag="LoxP(2)"
                     /label="LoxP(2)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2948..3004
                     /locus_tag="MCS (BsaI flanked)"
                     /label="MCS (BsaI flanked)"
                     /ApEinfo_label="MCS (BsaI flanked)"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3017..3316
                     /locus_tag="Right repair homology"
                     /label="Right repair homology"
                     /ApEinfo_label="Right repair homology"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 tagtaagcta tggaaaataa aagcggtaac tcgttcgagt tgagtctttc tacataattt
       61 acgaattttt tgggattttt gagaaaaatc cttgtttaaa attcaacttt gaagatttac
      121 aataataaaa taatacccga aaaaaacaga aaaataaaaa tgaacaaaaa acaacaaaat
      181 ctgaaaattg aaaaaaatag tgtctagcgg ggcacacttg catacattct gtctgcgtct
      241 cttcgcttca acatactgtc tcgacagtaa ttttcttttt caaagttttt tatttcattt
      301 taattcatta tttccgcttc aaaaagtaat ttgatatttt tttagagcaa aaATGATGTC
      361 ATCGGGACCC TTGGATCCAT CAGATGTCAC GTTATTCACT TCATACCCCA GTCCCATAGT
      421 TGTTCCGCTC GAATTCATAG AAGAACAGTC TAAATCGTCG AGAGAAGAGC TGGAAATTGA
      481 TGAAAGCGGT CTTTATATTA TGTCGAAAGC ACTCgtaaat tggaaaaatg ttgctcgctt
      541 cgttatattc tatttttcag GAAGAATTCA TGCGAACAGT GATGCGAGGA GCAACGCAGA
      601 ATGGTTCCCG ACTTCTAGAC TACGATTCAA TTGCCGAGTT CTTTGCCAAT TCAGAGTTCA
      661 GGGCGCTGAA AGgtaagtcg aattaaaaaa aaattgagaa aataaatacc gagttttttt
      721 cagATTTCTT CCCTGAAAGA GTCCGGTTCG ATGAAATTGA AAGCGAAATC TTACCAACTC
      781 GTGCCACAGG ACCATCTAAC TCTTCATGAc ttatttttta cagtaattct gtgatgcaca
      841 ttttccaaaa agattcacgt ttttttttga ataaacaatc ttaactctca agatcaaatt
      901 acgttgccat aacttcgtat agcatacatt atacgaagtt atttatgcaa gcaactttga
      961 gccaatttat ccaagtcctt gtaaaaagta ttcgaaaatt gttaacggat aaaatgttta
     1021 ttataatatc aaaaacaatt tgtcagttga ccactttttg atatatttga cagaaaccgg
     1081 gatgaattgg ctcaaagtta gggcgccctt ctattacagg gtttctgata acaaaccggt
     1141 tattaactcc caacaaggga tgatttcaat tcatcatgct caaattgacc caaattaagt
     1201 tacatgacaa attcatcgcc cttttcaact cttcttggct catcatctgt tattctgttc
     1261 ctattctgtc tgcaccctat acccctttgc atactctctc gtcattcctt tcttggatag
     1321 tgtcttcttc tccccagctc tgctacttct atgacttgcg cccgctgctt ttccgctcgt
     1381 tcctctcctc tctgacgtct tcttctgctt cttttctcct tcctttttcc cttctcaatc
     1441 ttcctctttt tcccatttct gtcaatcatt cgaagaagaa gaagaagaag accctcattc
     1501 atttcatttt tttttctgtc ggtgtgtgct gctcggttaa gagtgagctc tctattccac
     1561 gtcttcttct ttttcttctt cgattcgaat caatcactcc accaaaacgc attcgttttt
     1621 gggattcacc ccgcggttcg caaTaggttt ctttttcaaa ttattagcgt tataaataga
     1681 aaaatggtgg agtttcaata aaaatgataa tttacaaagt gtattttgat tacatgtact
     1741 caaaaaggtt gaaatttttc ataccagttt tccggaaatc catctgatat cattatcgta
     1801 tttttctttt taaaaatgtt ttcaaaaaaa aacaaaatat agctggttat ttggcaccct
     1861 ctaattacca ttttcctgtc acaccacact cttttccttc ttccacttct ttccgttttc
     1921 agccgcttcc aaccaaaccg atATGAAAGC CGAGCAACAA CGATCGATTC CACCCGGCTC
     1981 GGCGACCTTC CCGTCGCAGg tgagactcag aaaactagag aaaccgctca actaactctt
     2041 gatatccgat ttcattcttt tctcttttct ttttgttgaa cttttccata tttccagATG
     2101 CCACGGCCAC CACCAAGCAC CGAACAAGGA ATCACAACGG AATCGGAGCT TGCGAAGAAA
     2161 GCTCAAATCA CTCCGAACGA CGTTTTAGCA CTTCCGGGAA TCACTCAAGg tatcttgctc
     2221 cctttggatt tcagtttaaa acataaattt agGATTCTTA TGCTCCCCAT CTGCGAACAT
     2281 CTATAACATC GAGTTCACCA AGTTCCAAAT CCGTGATCTG GACACTGAGC AAGTGCTTTT
     2341 CGAGATCGCC AAACCGGAGA ACGATCAGGA GAATGATGAG TCGCCACAGG AGTCGGCAAG
     2401 ATACGTGCGT TATAGATTTG CTCCAAACTT TTTGAAACTC AAAACGGTCG GAGCAACTGT
     2461 GGAATTCAAA GTAGGAGACA TCCCAATCCA TCATTTCCGA ATGATCGAAC GTCACTTCTT
     2521 CAAAGATCGC CTTCTGAAGT GTTTTGACTT TGAATTCGGA TTCTGTATTC CGAATTCACG
     2581 AAACAACTGT GAACATATCT ATGAGTTCCC TCAACTCTCT CAACAACTCA gtgagtcatt
     2641 attctaaaaa gtacaattca aaagactaat ctctttttca gTGGACGACA TGATCAACAA
     2701 TCCAAACGAG ACTCGTTCTG ACAGCTTCTA TTTCGTCGAT AACAAACTCG TCATGCACAA
     2761 CAAAGCCGAC TACTCATATG ATGCATAAat cttcattgtt gagtttatct tgttgatttt
     2821 tgaataaatt atcaactctt tactttttaa tgggttatga aataaataaa cattgaaaac
     2881 tgataaacaa cgttcatctc tataacttcg tatagcatac attatacgaa gttattgcct
     2941 gagaccaGCT AGCAAGCTTG CATGCCTGCA GGTCGACTCT AGAGGATCCC CGGGTACCGA
     3001 GCTCaggtct ctgagctcag gaaacggaga atctgtgcgg caatgtctta tcatttgaaa
     3061 cgcgccgtat cgatgctgac atcatgcggc tcccgtgtta gtcgagaaga tgggtacggg
     3121 cctttattca tccaacactc tacggcttct tcaagagcta gtagggcacc ctgtagttgg
     3181 aaagggatta tttcgtatgg cgagcccata ccgtctttct tgcggaagct taacacgata
     3241 ggaagttgga tagtttcgaa cgatggttcc atacggaacg ctgtctcaag gatgagtctg
     3301 acggagtgta actcgagagc CAGAacctgg acccgtaaca ttttaagcca gtatttaaaa
     3361 gaacaaaata caaacaatca tttattcgag tttaataagt aatcaTTATC CCGATGACGA
     3421 TGCATTCTGG ACAGTCGAGG GTATACCAAA CATTTGTACA ATGTCAGGAA TTTCCAAAAA
     3481 GTAGACTTTA TCACGCATAG ATGCTACACC GAAAAAGAAG AATATTCCAG AGAAAAGAAT
     3541 AGCTCCAACG ACTCCACCCA ATCCACACAT GAAATCGTGT ACctagaaaa actaattatt
     3601 tttgtatatt gagacttcaa atataagctc ttaaaacatt actgtagcta tattatggtt
     3661 attatcgacc agtttttaga ttttacgaaa atacaggcaa aattacagta cagcaatgcg
     3721 actctcgcca cgaatgaaat gttttaaaat gtagttcgga aaaggtgtgc gcctttacac
     3781 agtaacccaa ctttagtagt ttcgcaattt agaacattct atctcaaaaa caactttcgt
     3841 tgaaaaaata aacgaaaaag aaacattccg aaataacgaa gaattggtta ctgtatcaat
     3901 taaaggcgca aaagtttgtt cgtggcgaga ccgggtaccg taattttggg cgaaaacacc
     3961 ataaatgcgt cataatattt ttccttcgta cCATTTTATT TTCAAGTGTT TCTGAACTTC
     4021 TATTGGTTCC TTGCTGGGCA TCAGCCACAA TCTTCGGTGC TTTGACAGCA CCATATGTCG
     4081 AAGCTTGAAA CATTctgaaa atgtattagt gagggttagt caagtggatt catcagtcaa
     4141 acatacTGTG TTGTGACCTC ACTAGTTTCA CCAACTTTAC CACCAGTTGC GGTATTTTGG
     4201 GCAGAAACAC CCGGGCCTGA AATACCCAGT GCTGATGGTC CTGCAGCTTT ATTGCTCGCA
     4261 GATGATGTTG CCATttttga aatgatcgga attgaggaaa aatcggaaat gattgaaatt
     4321 catttgatga atagaacagt ggattttc   
//
