LOCUS       mosTI_chrI_genom        7630 bp    DNA        linear       24-JUN-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >I I:7453386..7461015
COMMENT     
COMMENT     Target sgRNA for inserting landing site
COMMENT     TTCCGTGTCTTACTACTGTA
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(join(4202..4296,4346..4492,4557..4814,
                     4886..5111,5159..5232,5280..5415,5459..5713,6096..6302))
                     /locus_tag="spe-4"
                     /label="spe-4"
                     /ApEinfo_label="spe-4"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4063..4201)
                     /locus_tag="3'UTR spe-4"
                     /label="3'UTR spe-4"
                     /ApEinfo_label="3'UTR spe-4"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#f76ca1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(2030..2184,2236..2404,2456..2974,3065..3139,
                     3185..3473,3553..3713)
                     /locus_tag="lars-2"
                     /label="lars-2"
                     /ApEinfo_label="lars-2"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           3714..3869
                     /locus_tag="3'UTR lars-2"
                     /label="3'UTR lars-2"
                     /ApEinfo_label="3'UTR lars-2"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#f76ca1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3678..3927
                     /locus_tag="LH chrI"
                     /label="LH chrI"
                     /ApEinfo_label="LH chrI"
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="#ccff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3928..4111
                     /locus_tag="RH chrI"
                     /label="RH chrI"
                     /ApEinfo_label="RH chrI"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3911..3930
                     /locus_tag="sgRNA insertion"
                     /label="sgRNA insertion"
                     /ApEinfo_label="sgRNA insertion"
                     /ApEinfo_fwdcolor="#6666fe"
                     /ApEinfo_revcolor="#6666fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tctctctctc tcaatctggt gaccattttt ctcagaagag ctcttttccc ccacactgtc
       61 gttcgaggtt ttttctcaaa attttaattt ttaatttaaa cttgcagcag agcttatcca
      121 tcgtattagt tttatcattt aaataaataa ctttttttca aataacccag acatctcttg
      181 tgtaagaatt tttcagttat cgctaatctt cactactttt ctaaagttcc aaacggttaa
      241 aaatttatta aacatccaga aaatgttacg atggggtacc attcagcaga tccgtcgaat
      301 tggatcagca cttcagtggc cgttgaagga catccggatc agtgaaaata caaaaatgct
      361 ggagaaacat tggtcaaaat cacttgtcga ttcttttgaa tcttcaaaaa atgtagggaa
      421 ttttcattta taattgagtc tatagattat aataaaaatt aggaaaataa atttagaaag
      481 tttatttcta aactacaatt aatattttta gaaacccagc aatctcaatg gaaagaaata
      541 tattctatca atgtttccat atccatcagg acgacttcat attggccata tgagagttta
      601 tacaatctca gatgcaactg ctcggtatta tcgtttaaat ggatatgaag tgatacatcc
      661 gatcggatgg gactcttttg gattaccagc agagaatgca gcaagagaca aaggagttga
      721 tccaagagaa tggactatta ataatattga atcaatgaaa caacaactct taaaaaccaa
      781 gattttattt gattgggaga aagaaatttc aacgtgtgaa cctgaatttt tccgttggac
      841 acaatggatt ttttgtaaac tttttgagta cggactggtg aaacgaacat cttcggaagt
      901 taattgggat ccaatcgata aaactgttct ggctgctgaa caaatagata acgatggaaa
      961 aagttggaga agtggagcga aagctgaaaa gaagaagctt agccaatgga tgattgaaac
     1021 gccaaaatat gcaaaaagac tacaagaagg tttaagaaaa atgtctgaac aatggggaga
     1081 agttgctgat attcaagcta attggatcgg aaaatgtgat gtttatcggt ttatgttgcc
     1141 tgttatgaag gatggatcaa acgatgaaat tattgatttg agaatcaaag atatctttga
     1201 aatttcaaat gccgaatttt tggttttgaa gcaaagtcac tcgttggcag acaaggatgc
     1261 cacgcagttt ccatgtaagt tctcgtttca tcggaaagta attgttttaa ttattggaac
     1321 tgaatctaga atttatttat ggaaatttca aacaaataat atactcgctc aaattttcag
     1381 acaaacttcc gtttcaagtt ttgaacggag taacaggccg tcaaatacca gtcattgtag
     1441 tggaagacac atatcaacca gatgtcacaa gcttcttgaa ctcgagaatt ggaaactttc
     1501 agaaagacaa aattcttgtc gaaaagtttg cgatagctga gccgaaacac aaaagaaagt
     1561 tgacgaagaa tgatattcag gagatggcat cggtaacaca cgattacggg atccggtcac
     1621 gacgcgactg aaaatttttc cgtgaaagag tgtgcgcctt taaaaagtac ggttcactac
     1681 atccagattt tcgcaatttt tatcgatttt tcatcgactt ttcccttaaa gccaaccaaa
     1741 aacaggaagt tgcaattaca gtactcgaga aaggtataca ccgtttttct cagaaatttt
     1801 cagccgtttc gagaccggat accgtaattt ttcctcaaaa atcgcatttt gtagaaaatg
     1861 gataataagc gaatgtgtta ttttctgcaa ctttttcaag aaaaatattc aaaaagcgtt
     1921 cacaaatgcc aacaattttc agtttggtgg atacggtggc tatgaaacat cccgaacgtt
     1981 aacagattgg gttgtgagtc gtcaacgtgg atggggaact ccaattccaA TGATCCAAAC
     2041 CAAAGACGGA AAACGAGTTG CTGTTCAATT AAATAAACTT CCAGTCCTTG GGTCAGATCG
     2101 CGGGCAAGAA GTAGATAAAG AAAGATTCGG AGAATCCGGA GTATTCGACA CAGATACTCT
     2161 TGATACATTC TTTGATTCTG CATGgtagag tatatatctg aaatgtacat ttggattaaa
     2221 cttgaatatt tttagGTACT ATCTTCGATA CCTTGACAAC AAAAATAAAA GCGAATTGAT
     2281 TTCCAAAGGA GCAATGAAGA ATATGCCTGT TGATGTCTAT GTTGGAGGAA TTGAACACGC
     2341 AGCGGTGCAC ATGTTTTTTG CTAGATTTGT TGCATATTTC TTGAAGGATA TCAATGTTAT
     2401 CCAGgttggt aaaaactatc atttttaggt ttaatcaaaa tttaacattt tccagACAGC
     2461 TGAACCATTC ACTGACCTCA TCCCACAAGG AATTGTTCGG GGAAAGACGT TCATCGAAAA
     2521 AGAAACAGGA AAATATCTTG GATCAGAAGA TGTTCAACAA TCTGGAGATA AACTCACATT
     2581 GAAGAATGAT TCATCTGTTG AAGTTGACGC TGTCTATGAA AAGATGAGCA AATCGAAAAA
     2641 CAACGGCGTA GATTTGGCTG CAATTTTGGA TTCGGAAGGA GTTGATATGA CAAGACTTAG
     2701 ATTACTTGAA GCTGCAGCTC CGAGAGCTCC CATTAATTGG GGCGAAACAG ATTTAAAGGG
     2761 AATCAAGAAA CTTTTGGATC GAATTGCGGC AATCAATTCG CAAATTGTTG ATGCACGAGA
     2821 TAATGGAAAA CCAATAGAGT TGAAGAAAGA AATTGATGGA AGTATAAAAG AAACATATAA
     2881 CTTTTTCGTA AGAAATGTTG GAATGTGTCT AGAAGTACTT CATCTTCATA ATACTGCGTT
     2941 GATGAGAATA CAGGGATTCA CTAATGCATT AAAGgttatt taacaaatat ttttaaagtc
     3001 tgaaatgatg ttttgatatc actttttaaa gaaaaaaacc acaaactaaa atgatttatt
     3061 tcagAAAGTT GATCCAATCT ACCTGGCTCA CAGCCCAGAA GGCCAACGAG CGGTGAAATC
     3121 GTTGATTATT ATGCTTCAAg tagtttttaa gttctaaagt gcaatctgtt cattaatttt
     3181 ctagGTTTTT TGTCCACACG TCGCTTCCGA AATGTGGTCA GCATTAGCTA ATGATGGTTC
     3241 ACTGGTTTCC GATCAAAAGT GGCCCGAAGT TGATAAAGAT GCTGATATTG AATTTCTTCT
     3301 GATGATTGAT GGTGTTAGTG GTGGTAGGCC TTCTCTTGAT CGGCGGATTA TTGAAGAAAT
     3361 GTCTATGGAT CAGTTATGGG AACGAGCTAC ATTTTATGAA CACGCAGATA TTCTTCGAAT
     3421 GATGAAAGAA GATGGAATGA AGCTGAAAAA TTCAAGTTAT ATTTCTAGGA AAGgttaggt
     3481 tttgtctgtc tagttaccaa ctttagatta caataagaaa tctaagttat taagttatta
     3541 acaaattttc agGTTTCCAC GTAACTCTAT CGTGTAGCTT GGAAGGTGAC AAAGAAGAGA
     3601 ACCGCAAGAA AATTGGCAAA ATTCTGGACA GAATTCAAGC AGAGAAGAGA AAGTTGGAAA
     3661 TCGGGAAGAA AGGTGGAAAG AGGAAGAAAG AAAAGAAGAT TGTGAATAAG TGActtattt
     3721 tactggagaa cattagttta atttttaaca gttttgaaat atatattagc ttagaaatag
     3781 gttgttgttt tattgttttc tgaataattt ttatatttac gggcttgttt attgtttttc
     3841 atgaaaataa atttgttgag tgactccaac ttaccagctt cgaaattgaa tggacgaaac
     3901 gcaattcgcg TTCCGTGTCT TACTACTGTA AGGtatttac gataaaagaa tggaaatttg
     3961 tcgattatat acgatttcgt gttttcagta taactaaaaa attgagtata taaaccatcg
     4021 agacaagtct ccttgattac tgtaattttc aacctcatta ctaatacgat actatgtatt
     4081 ctttgccaaa tagattcgta ttcataaaat taaaaaaaaa aacgaaaagg ttaaaattaa
     4141 tcattcagac aagtaaataa gacatttaat cagttcacat aatatcagga aaaatgaggc
     4201 tTCATCCGTA AAGTTGCTCC CAGAAAAACA TTGAACTGAA ATAGCAGAAA GTACCACAAA
     4261 TCACAGGCAA CGGCAGAGCA GGAGTTGTGG ATTCCTctga aacgagacaa ttagtattga
     4321 acttcgtgat tcatatggtg attacCAGTT GAAAAGACAG TGAGAGTCAC AACAAGTCCA
     4381 AATAAAATAC CAAGAGCGGC AGAAATGACT GCAAATGGAC ATCCGCTGGC AGCCGCTTGA
     4441 CCAATCAGAA GACTGTAGAA GACGAAATCT CCAAAGCCGA GACGAAGTAC TTctgaaact
     4501 ttctcgtaaa aaaagctggt aagagagtaa gcttaatatg acgaaactat tattacCTCC
     4561 ATCATTAAGG GCGTCCGCAG CAGTGGCTGG CCGTTTATCA TTGTTCGGTA GACTGTTAGA
     4621 GACCAAATCA TCCCACTCCT TCTGATCACA TTCCTCAGCA GTGCTTATAT CAGATGTTGA
     4681 CACAGTGGTG CTTGAGTCGG AAGACGATAA ATTAGATGAT CCACTTGAAG TTTCAGATGT
     4741 ATCACTCTCA TCATCACTGA GCTCCTCGGT ACTGTTCTTT TCCTTTAATT CGATTGGTGA
     4801 TGGCTCGGCT TCTActgaaa aagaaaaaat gaataatagc aactgtcatt cttataaaga
     4861 tctaaatgat ttatttaatt cttacCTAAT GGGCTATGCT CAGTTGGTAC CGAATCTGGA
     4921 TTGATTGCAG CCCGACGTTG TCTGATCTTT TGATAAAATT CATCATCTTG AGCTTCACGT
     4981 TTTGTATAAT ATTCAATCGT TTGCTTCACG GTTCTTCTGA TTGTACTCTC CTCTCCTTCA
     5041 TTTGTCTCTT CTTGATTGGA TCCTGCAGTT AAACGTTTTT CATTAGCAGA AAACATAATT
     5101 AAATTGAGAA Cctgaaaatg aaaataatca gatttatttt gaaaattgct attcttacGC
     5161 ATTTACTGTA GTCTGAAGCC TTTTCTTGAA CTTTTTTGAG TGGACCCATC GGTGCTAAAA
     5221 CGGCAAAGAG ATctggaaaa acttaattaa atgcataaga gcacaggaag aaaacaaacC
     5281 CCAAAATAGA ACAATCCAGA GAACAAACCA AGTGGTTTTG CTTGGAAAAA CTCGTAGGTA
     5341 GAAAACGGAG ATAAGAGAAC AGTTTGTGAC AACGAATATT TGATGAAGAA TCAAAGAACT
     5401 GTTAGAGAAG AATGCcttaa aataattatt aatttttatt tggttacata acacttacAT
     5461 AGATCCCTCC GAACCCATAT ACAACCGTTG GAACCACTAT CAACACAATT GTCATGTAAT
     5521 ATTGATTGTT GTCATCTTGG TCAAATACTT GTGAAAACAT ATCATGAAGA GTCTGCGCGG
     5581 ATACCCCAAA CAATATCAAA AGACATGAAA GTGTGAGCCA GGCTTTTACG ATACGACGGA
     5641 AATCAAAGAG TACGAAAGCG AGCATTATGA AAGAGACGCA TCCAAGAACG AGAATTGTAC
     5701 CAACTCCATT GATctgaaat gaaaatatga gaactgaaag tggaagagag tattaaatta
     5761 ttataatgta cgagtggcaa acttttttga taaactacaa agaaattcaa tttatgcttc
     5821 gtgcgaaacg tagacaaata ggtaggcaag taggtaggta gaccaccgga agacgtgtct
     5881 tcctatttct tgccggtttt taaaatgata aaagtattca acttcttgtt gtgaaatgta
     5941 atgttggatg tctcttttct tgaattgcag gatcaatccg aaataatcaa gctccaaaac
     6001 atttggggaa tcatgcccag aaataactct agtaaattaa gaattaaaat gttattctct
     6061 aactcaaaaa ctcagaatgt gcagtccaaa ctcacAAATC CATCCAACAG CAAATTTCCA
     6121 GTTGTTTGCT CAAACGAAGG ATCCAAAAAA TAAGTCTTGC TCAATTCAGA ATTCACTTCC
     6181 ATGTTGTAAA CTCCAATCCA AATAGACAAT GTCAGTGACA TATTGGCAAT AACAGAGAAC
     6241 AGTGTCCATC GTAATTGTGA AGATCGCACT AATTCGCTAG AAATCGATCG AAGGGTGTCC
     6301 ATttttagac aggtaataaa attaatatta gcacaagaga tttgtatgaa attgatgagt
     6361 tggcagacaa aagtatgaca cgtgcatgtt tgtactttgg tttggatcac ggggtcacga
     6421 tgaaatcatt ttaaaatatt attttggaag aatattgccg tttctaacat tgaagtgaaa
     6481 aattgaacaa gcattgtaca tgtcgtgctt ttttctagaa atgctctctt tcagagcttg
     6541 atcagaacaa ccactctaca gtactccaat tcgtttcgaa aaacccttct tctaggatcc
     6601 taagcttctg gtgcgtcctc ttctggacgc gtcctcttct gggcgcgtcc tctggagcac
     6661 accgagtttg gaaaaattta ataaaaattt ttaaaccagg agatcacgca aaaagggttg
     6721 aaacatagcg cgctgagatt gagcgctaga ggatatcaaa agcaaccgtg ttactctagg
     6781 gtgatccaaa acgaaatttt gaatagtaaa atatccaaaa attgtcttgt attgcaactc
     6841 acattgtaag taaactcctg aacaaagaaa ttttcaaata aactacttga agtgtttcct
     6901 ttactgacat gaatgtttat acttaaaact ttataaaatt caaaaattga atttgaaagg
     6961 cacatggaaa agcaacggtg aagtatagaa agtagtgatt gattgattgt gagagggata
     7021 aaaagggata aattttgaga cgtttaacct gaaaaagaaa acaaaacgaa agaagagaga
     7081 tgaaaaaaat ctcgtcatat tcttgacttt tggtcaaaga gaacccattt ggttttgaag
     7141 gggaaagaag tgatgaaaaa tttcagaatt cgaggaaaga aaaaaaaaac gacaaaatgt
     7201 acaggtattc gtagaggaga tgaaaattga tcgaatgaag aacaactcta ttgctttgaa
     7261 tccaacaaaa aaaggcactt gaatcactat gttcgattga tgttttgact tgatgctgat
     7321 gcagccatta ctgtttctgt ctcgtatcgg cattccgttt ttaggcgtac aaagtccttt
     7381 gccacctgta aaaatgaaaa atgattattg aatttttttt ggaaatatgt gagtttctaa
     7441 aaacgctcat ataatgattt aacaggacca aataccataa ttaaacactt caaaattttc
     7501 agtcaaagtt tcgacaaact gccaaattgt ggaaaaattt tttagagagg gtcttataaa
     7561 taatttcttt ggtcgctttc ctgaattatg tgaaaactaa aatttcagat ttttttctca
     7621 agcttttgaa     
//
