LOCUS       mosTI_chrII_w_la        8006 bp    DNA        linear       24-JUN-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(838..957,1002..1155,1199..1353,1578..2140,2454..2603,
                     2653..2929)
                     /locus_tag="F14E5.1"
                     /label="F14E5.1"
                     /ApEinfo_label="F14E5.1"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5479..5481
                     /locus_tag="pam1"
                     /label="pam1"
                     /ApEinfo_label="pam1"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           2930..3001
                     /locus_tag="3'UTR"
                     /label="3'UTR"
                     /ApEinfo_label="3'UTR"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#f76ca1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(6034..6120,6424..6481,6979..7094,7180..7284)
                     /locus_tag="F14E5.8"
                     /label="F14E5.8"
                     /ApEinfo_label="F14E5.8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5160..5459
                     /locus_tag="Random sequence - no in frame stop and no
                     polyA consensus"
                     /label="Random sequence - no in frame stop and no polyA
                     consensus"
                     /ApEinfo_label="Random sequence - no in frame stop and no
                     polyA consensus"
                     /ApEinfo_fwdcolor="#66fffe"
                     /ApEinfo_revcolor="#66fffe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           7285..7325
                     /locus_tag="3'UTR(1)"
                     /label="3'UTR(1)"
                     /ApEinfo_label="3'UTR"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#f76ca1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5129..5148)
                     /locus_tag="sgRNA insertion"
                     /label="sgRNA insertion"
                     /ApEinfo_label="sgRNA insertion"
                     /ApEinfo_fwdcolor="#fc6fce"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3130..3379
                     /locus_tag="LH ttTi5605 (Esp3I)"
                     /label="LH ttTi5605 (Esp3I)"
                     /ApEinfo_label="LH ttTi5605 (Esp3I)"
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="#ccff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     3398..3431
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3363..3379
                     /locus_tag="ttTi5605 sgRNA 1/2"
                     /label="ttTi5605 sgRNA 1/2"
                     /ApEinfo_label="ttTi5605 sgRNA 1/2"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5476..5481
                     /locus_tag="ttTi5605 sgRNA 2/2"
                     /label="ttTi5605 sgRNA 2/2"
                     /ApEinfo_label="ttTi5605 sgRNA 2/2"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3440..5114
                     /locus_tag="partial cbr-unc-119(-)"
                     /label="partial cbr-unc-119(-)"
                     /ApEinfo_label="partial cbr-unc-119(-)"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(4428..4484,4583..4694,4738..5114)
                     /locus_tag="cbr-unc-119 exons 3/4"
                     /label="cbr-unc-119 exons 3/4"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5126..5128)
                     /locus_tag="PAM"
                     /label="PAM"
                     /ApEinfo_label="PAM"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5476..5722
                     /locus_tag="RH ttTi5605 (Esp3i)"
                     /label="RH ttTi5605 (Esp3i)"
                     /ApEinfo_label="RH ttTi5605 (Esp3i)"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcattaaatg actataaatt ttttaacaac gtaagactag aaatttcgaa aagggacgcc
       61 tcttcgctcg cctttctttc catgatttcc aattaaaagc acttggcgat taattttgta
      121 agggtgcaac cagtttatag tagaatccac aaaagatcgt gtgctcagga ttgaatagga
      181 agttaacatt tttagagaaa ccactagagt gttttcttaa ctttggaagc ctttccaaag
      241 cactgtgtat taattttaaa tgttttttct ttcaaaagta taaccaaatg atgtcattga
      301 cacctcctca aatacttcaa atacagaaca cctacacatt ctcatttgaa ctttctttgc
      361 ggtagcatcg acgacctttt ttggtctctt tctctcactt tcaagattgg tgtaattcaa
      421 aaaagaaaag cgagaaattt gcaaatggca acaacaattg gcgcaagaca ctggacgcta
      481 atcaagggtg acacaagaga acgagatgaa gattgatggc cttggaacgg aaactcattt
      541 tgaaaaaaaa ttttgaataa ttagacaatt aacataggtg catgggaaaa ttgaaatcgg
      601 gctgatctgt tataatagtt tttcacagga atttgtgaag aacatttaga tttttttgcc
      661 atttaaactt tcatactgtt ttaatagtat tcattttcag gttttaatgg aattcatcaa
      721 taccctacac tgataaataa aacaaattgt agtcaactct gataatgcta ctgatggaca
      781 aagttcctcc gtattcattt tgtgcacttt gttgccattt cttgatttgg tttttaaATG
      841 TCAAATAGAT TGTGGCCTGT TAAACAAGTG TGTATTTTCG CACTTATTGC GTTTGCATCT
      901 AATTTTAATT ATGGATTTTC AACTACCTAT ATCAATACAT CTGTTGATGA ATTTAAGgta
      961 aatatgaaat acaaataaat ctcttcatat ttttgtttaa gACATTTTTG AATACATCTT
     1021 TAAGTCGTCG TCATGTCATA ATGACTGAAG AAAAATATGA CCTAATTTGG AATGTTTTTC
     1081 TGAATTGTTG GTTTATTGGA TCATTTTTTG GAGTTTGGAT TAACTCGATT TTTAGTGAAA
     1141 GATTTGGAAG AAGAGgcacg atttcatgaa ctataaaggt tcaaaatata gttttcagTG
     1201 GGCTTTTTGA TTGGAAATTC GGTTGCATTC ATTTCTTCAA TTTTACAATG CTTGTCTCTT
     1261 ATTTGGTATT GTCCTGAACT ACTGATCGTT TCTCGATTCA TTACGTCAAT TTGCATGGCA
     1321 GCTACTTATC AATCATGTAT TTTGTTTTTG CAGgttagta ccatatgatt tcttgatatt
     1381 ccatatctat ggattctaga ataattttag gaaaaaataa ttttgagacg caacattatg
     1441 cgaaaggaat gcgaaattcc aatagtaatt tccgatggtt tatttagggt tttagaatac
     1501 tgccattaat taaaaattat atctagtttc aaagtttctc tatttatctt gtatttctga
     1561 tataaacata ttttcagGAA TGCTCTCCAA CACATTTACG TGGTAGTTTT TCATTTCTCA
     1621 GTGAAGTATC ATATTCATCA ATGACAATGG TTGGAAGTTT TCTTGGTCAA GATTATATTA
     1681 TAGGATCACA TCTTTTCTGG CTCTGCTTCT TCGTTGTACC ATTTTGCCTT TTCTTCACAC
     1741 TTATTCTTTT CATACTTCCT GAAACTCCAA AATTTCTATT AATTTCGAAA GATGAAGAAG
     1801 AAGAAGCTAT CAAATCTGTA AAATATTATC ATGGAGATGG TACAGATGCA AAACAAGTTT
     1861 TGGAAGATAT TCGAAAAGAG GCAGAATGTG AACAAGACTC GAGCAAATCG ACAACTTTCC
     1921 AAAAAATGAA GGAATTGTTT ACAGAAAGAC ATTTGAGAAT GGCATTGATA TTAAGTGTAT
     1981 CTGCATTAAC CAATACTGTC GGATTATGGG CTCTTCTTTT ATCCTCTACT TTCTTTCTAG
     2041 AAAATGCAAA TGTTGAGTCA GAAATTGCTG AATGGAGTAC AACTGCAATG GGTTTAGCTT
     2101 ATGTTTCTGG AACAATTACT GGTGGAATTG TGATTGAAAA gtaaggttat atttcatttc
     2161 aataaatgtt accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt
     2221 attattatta ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata
     2281 atctcaacgt ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat
     2341 agttttatta gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag
     2401 atagttttaa acgttcaaaa tttgaagaca aaattgtatg aataagttta cagAGTTGGT
     2461 CGACGCAAGC TTCTCCTTCT CTTCACATTT CTCAATAATT TAGCTCTAAT TGCATTCGTA
     2521 TTCTTCGCAA AAATACGTAT TCTGATTGAC CCGATGAAAT ACGGATGCCT AGTTGCATTG
     2581 ATCATCTACG GTTATACTTA TGGgtgaggt ttttcaaata tattttgatt taatcgaata
     2641 atgaaatttc agAACTGGAG TTGGTCCAAT TTCGTGGTTT ATATCCTCTG AACTGGTACC
     2701 TCAAAAACAT CGTAGTATTG CTCAATCTGT GGCATATTCA ATGAATACTG TAATGGTGGT
     2761 TATCAGTACA TTTACAGTAT TACCTCTATA TTCCGTTATT GGTAGTTATG CTTTCCTTAT
     2821 TCTATATTCA ATTCCTTCAT TTATAAGTAT GCTTATATTA TTTCGCTACT TACCGGAAAC
     2881 CAAAGGACGA GAGATTCATG ATATTGTGAA TGAACTTAAA AAAAAATGAa aacctttgaa
     2941 aacgttcata cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac
     3001 agaaaagtga gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa
     3061 gaagaaaaat acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa
     3121 cttttttgca aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat
     3181 gaatatcaac aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc
     3241 gaatcagacc cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc
     3301 ctgattccat gatggtagca aactcacttc gtgtaagtgc aagtaagatc agtgtttgtt
     3361 tcgatatcag tctgtttcga cgtctcttta cgttgccata acttcgtata gcatacatta
     3421 tacgaagtta tgcaagcaac tttgagccaa tttatccaag tccttgtaaa aagtattcga
     3481 aaattgttaa cggataaaat gtttattata atatcaaaaa caatttgtca gttgaccact
     3541 ttttgatata tttgacagaa accgggatga attggctcaa agttagggcg cccttctatt
     3601 acagggtttc tgataacaaa ccggttatta actcccaaca agggatgatt tcaattcatc
     3661 atgctcaaat tgacccaaat taagttacat gacaaattca tcgccctttt caactcttct
     3721 tggctcatca tctgttattc tgttcctatt ctgtctgcac cctatacccc tttgcatact
     3781 ctctcgtcat tcctttcttg gatagtgtct tcttctcccc agctctgcta cttctatgac
     3841 ttgcgcccgc tgcttttccg ctcgttcctc tcctctctga cgtcttcttc tgcttctttt
     3901 ctccttcctt tttcccttct caatcttcct ctttttccca tttctgtcaa tcattcgaag
     3961 aagaagaaga agaagaccct cattcatttc attttttttt ctgtcggtgt gtgctgctcg
     4021 gttaagagtg agctctctat tccacgtctt cttctttttc ttcttcgatt cgaatcaatc
     4081 actccaccaa aacgcattcg tttttgggat tcaccccgcg gttcgcaaTa ggtttctttt
     4141 tcaaattatt agcgttataa atagaaaaat ggtggagttt caataaaaat gataatttac
     4201 aaagtgtatt ttgattacat gtactcaaaa aggttgaaat ttttcatacc agttttccgg
     4261 aaatccatct gatatcatta tcgtattttt ctttttaaaa atgttttcaa aaaaaaacaa
     4321 aatatagctg gttatttggc accctctaat taccattttc ctgtcacacc acactctttt
     4381 ccttcttcca cttctttccg ttttcagccg cttccaacca aaccgatATG AAAGCCGAGC
     4441 AACAACGATC GATTCCACCC GGCTCGGCGA CCTTCCCGTC GCAGgtgaga ctcagaaaac
     4501 tagagaaacc gctcaactaa ctcttgatat ccgatttcat tcttttctct tttctttttg
     4561 ttgaactttt ccatatttcc agATGCCACG GCCACCACCA AGCACCGAAC AAGGAATCAC
     4621 AACGGAATCG GAGCTTGCGA AGAAAGCTCA AATCACTCCG AACGACGTTT TAGCACTTCC
     4681 GGGAATCACT CAAGgtatct tgctcccttt ggatttcagt ttaaaacata aatttagGAT
     4741 TCTTATGCTC CCCATCTGCG AACATCTATA ACATCGAGTT CACCAAGTTC CAAATCCGTG
     4801 ATCTGGACAC TGAGCAAGTG CTTTTCGAGA TCGCCAAACC GGAGAACGAT CAGGAGAATG
     4861 ATGAGTCGCC ACAGGAGTCG GCAAGATACG TGCGTTATAG ATTTGCTCCA AACTTTTTGA
     4921 AACTCAAAAC GGTCGGAGCA ACTGTGGAAT TCAAAGTAGG AGACATCCCA ATCCATCATT
     4981 TCCGAATGAT CGAACGTCAC TTCTTCAAAG ATCGCCTTCT GAAGTGTTTT GACTTTGAAT
     5041 TCGGATTCTG TATTCCGAAT TCACGAAACA ACTGTGAACA TATCTATGAG TTCCCTCAAC
     5101 TCTCTCAACA ACTCtgccgg agacgccaGT GCATAATCAA CCTTCCCCcg tctccgcaat
     5161 caggaaacgg agaatctgtg cggcaatgtc ttatcatttg aaacgcgccg tatcgatgct
     5221 gacatcatgc ggctcccgtg ttagtcgaga agatgggtac gggcctttat tcatccaaca
     5281 ctctacggct tcttcaagag ctagtagggc accctgtagt tggaaaggga ttatttcgta
     5341 tggcgagccc ataccgtctt tcttgcggaa gcttaacacg ataggaagtt ggatagtttc
     5401 gaacgatggt tccatacgga acgctgtctc aaggatgagt ctgacggagt gtaactcgag
     5461 agccagaaga gacgttaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc
     5521 acaactgaga aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta
     5581 aaaaatcgag tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt
     5641 ctcctgggcg tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc
     5701 aattatttca tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca
     5761 tacaaacgag gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct
     5821 aattagctat ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt
     5881 ctaattagaa atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga
     5941 aatgatcccg cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt
     6001 acacaattca agttacatta ttttcaggaa acaATGGCGA AGAGCAAAGC AGAGATTCAA
     6061 AAGGAGGAAA GAGACAACAG ATATGAACGA AGACTTCTTG AAAAGAGAAA GAGACATGAG
     6121 gtgagtaacc atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt
     6181 aacagtttcg aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata
     6241 gcctaaacga gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt
     6301 tatcctttca aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt
     6361 tatatcatat ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg
     6421 tagATCGACA ACAAGGCCTC CACTTCTTCA TCGATGGCCT CTGCAGCACC TGCACCACCA
     6481 Agtttgtatt tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg
     6541 ccagatcgat gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg
     6601 gccacggatt gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac
     6661 aaaatatggt tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag
     6721 atattttggg tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac
     6781 acaaaaagtt cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat
     6841 cgccgtgttc aaaaacattt tcatgtattt tttataaagc tataaaagtg gacttttaca
     6901 gttaggttcg cctcccgatt tttcacattc cgctttttaa ggcacatttt agtgtttttt
     6961 caattatatt cttttcagTG AAGAATGGGA GAACACGCAA ACAGCAAAAA CTTCAAAATT
     7021 TGGCACTTGC TCGAGAGAAA CAACGCATTA AGAGGGCTGC AGCAAAACAT GTGAGAAACA
     7081 ACGATAATAA TCAAgtaagg gttatggttt cataaattct gaagaattaa cagttttgaa
     7141 aaaaatagtt taaaatttta aaactatcaa attttgtagG TCGACAACGA GGCTGATCGA
     7201 ATAGATGAAG GTGCCGCGAA TTCTGACGCT GGCAAAATTG GATTATTTCA AAAAACGGCC
     7261 TGTAACTTGG CTGAACATAA TTAAaccaaa aaaattgtac ctgataaaat gcggaaaaat
     7321 atcttgcaat tattattctt taacatttta cttctgaaac tcaatcgaca aaatttttag
     7381 tggtttttgg taaaatgact ctgtgtaact cgacaaataa ttggtacttt gcaatgaaaa
     7441 gtaatgcaga aaaaataaaa aatatttcta atcattttgt tagttgaaca tttgtttcct
     7501 agatgtttat ttttcaagat acgccagttt gaagatacgc aatttcgtga gttcaaaaag
     7561 ttatttggta aaccgagcct gggcttgcaa cttttgctgg caagtattat tttaatgaga
     7621 atttagaatg ttgtattgcc taactattga attgtagaaa attatagtat acttctataa
     7681 cggttcaata aaacaatagt gatgtactgt gggcacaggt gtcacagtgg atttgtagtc
     7741 caaaatgact gcctgaaaaa acaacattat ttttgagctt caaaactaac ttgcaagtag
     7801 gtcaaaacgt tcagactgag ccagtcttac tccaccccca ctaccatcct cacaagtgaa
     7861 cgaattccgt caactaggta taacttattc atttgtacat tgaacatttg ctgcagcaat
     7921 gcaagttctt gatgatataa cagctgaact agcgaatcta gatcatcttc gtaaacgttc
     7981 catccgtgaa atacaaaata cattgc   
//
