LOCUS       3__mosTI_chrI_la        8063 bp    DNA        linear       24-JUN-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     kstSi32 targeted with empty pSEM246 (your exact configuration will
            depend on what you cloned into MosTI targeting vector)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(join(4635..4729,4779..4925,4990..5247,
                     5319..5544,5592..5665,5713..5848,5892..6146,6529..6735))
                     /locus_tag="spe-4"
                     /label="spe-4"
                     /ApEinfo_label="spe-4"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4496..4634)
                     /locus_tag="3'UTR spe-4"
                     /label="3'UTR spe-4"
                     /ApEinfo_label="3'UTR spe-4"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#f76ca1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4053..4352
                     /locus_tag="Random sequence - no in frame stop and no
                     polyA consensus"
                     /label="Random sequence - no in frame stop and no polyA
                     consensus"
                     /ApEinfo_label="Random sequence - no in frame stop and no
                     polyA consensus"
                     /ApEinfo_fwdcolor="#66fffe"
                     /ApEinfo_revcolor="#66fffe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(2030..2184,2236..2404,2456..2974,3065..3139,
                     3185..3473,3553..3713)
                     /locus_tag="lars-2"
                     /label="lars-2"
                     /ApEinfo_label="lars-2"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           3714..3869
                     /locus_tag="3'UTR lars-2"
                     /label="3'UTR lars-2"
                     /ApEinfo_label="3'UTR lars-2"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#f76ca1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3911..3927
                     /locus_tag="sgRNA insertion"
                     /label="sgRNA insertion"
                     /ApEinfo_label="sgRNA insertion"
                     /ApEinfo_fwdcolor="#6666fe"
                     /ApEinfo_revcolor="#6666fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3678..3927
                     /locus_tag="LH"
                     /label="LH"
                     /ApEinfo_label="LH"
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="#ccff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4361..4610
                     /locus_tag="RH"
                     /label="RH"
                     /ApEinfo_label="RH"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3984..4040
                     /locus_tag="MCS (BsaI flanked)"
                     /label="MCS (BsaI flanked)"
                     /ApEinfo_label="MCS (BsaI flanked)"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4357..4360
                     /locus_tag="MoClo-5F-Esp3i"
                     /label="MoClo-5F-Esp3i"
                     /ApEinfo_label="MoClo-5F-Esp3i"
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4361..4363
                     /locus_tag="sgRNA insertion(1)"
                     /label="sgRNA insertion(1)"
                     /ApEinfo_label="sgRNA insertion"
                     /ApEinfo_fwdcolor="#6666fe"
                     /ApEinfo_revcolor="#6666fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     3938..3971
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tctctctctc tcaatctggt gaccattttt ctcagaagag ctcttttccc ccacactgtc
       61 gttcgaggtt ttttctcaaa attttaattt ttaatttaaa cttgcagcag agcttatcca
      121 tcgtattagt tttatcattt aaataaataa ctttttttca aataacccag acatctcttg
      181 tgtaagaatt tttcagttat cgctaatctt cactactttt ctaaagttcc aaacggttaa
      241 aaatttatta aacatccaga aaatgttacg atggggtacc attcagcaga tccgtcgaat
      301 tggatcagca cttcagtggc cgttgaagga catccggatc agtgaaaata caaaaatgct
      361 ggagaaacat tggtcaaaat cacttgtcga ttcttttgaa tcttcaaaaa atgtagggaa
      421 ttttcattta taattgagtc tatagattat aataaaaatt aggaaaataa atttagaaag
      481 tttatttcta aactacaatt aatattttta gaaacccagc aatctcaatg gaaagaaata
      541 tattctatca atgtttccat atccatcagg acgacttcat attggccata tgagagttta
      601 tacaatctca gatgcaactg ctcggtatta tcgtttaaat ggatatgaag tgatacatcc
      661 gatcggatgg gactcttttg gattaccagc agagaatgca gcaagagaca aaggagttga
      721 tccaagagaa tggactatta ataatattga atcaatgaaa caacaactct taaaaaccaa
      781 gattttattt gattgggaga aagaaatttc aacgtgtgaa cctgaatttt tccgttggac
      841 acaatggatt ttttgtaaac tttttgagta cggactggtg aaacgaacat cttcggaagt
      901 taattgggat ccaatcgata aaactgttct ggctgctgaa caaatagata acgatggaaa
      961 aagttggaga agtggagcga aagctgaaaa gaagaagctt agccaatgga tgattgaaac
     1021 gccaaaatat gcaaaaagac tacaagaagg tttaagaaaa atgtctgaac aatggggaga
     1081 agttgctgat attcaagcta attggatcgg aaaatgtgat gtttatcggt ttatgttgcc
     1141 tgttatgaag gatggatcaa acgatgaaat tattgatttg agaatcaaag atatctttga
     1201 aatttcaaat gccgaatttt tggttttgaa gcaaagtcac tcgttggcag acaaggatgc
     1261 cacgcagttt ccatgtaagt tctcgtttca tcggaaagta attgttttaa ttattggaac
     1321 tgaatctaga atttatttat ggaaatttca aacaaataat atactcgctc aaattttcag
     1381 acaaacttcc gtttcaagtt ttgaacggag taacaggccg tcaaatacca gtcattgtag
     1441 tggaagacac atatcaacca gatgtcacaa gcttcttgaa ctcgagaatt ggaaactttc
     1501 agaaagacaa aattcttgtc gaaaagtttg cgatagctga gccgaaacac aaaagaaagt
     1561 tgacgaagaa tgatattcag gagatggcat cggtaacaca cgattacggg atccggtcac
     1621 gacgcgactg aaaatttttc cgtgaaagag tgtgcgcctt taaaaagtac ggttcactac
     1681 atccagattt tcgcaatttt tatcgatttt tcatcgactt ttcccttaaa gccaaccaaa
     1741 aacaggaagt tgcaattaca gtactcgaga aaggtataca ccgtttttct cagaaatttt
     1801 cagccgtttc gagaccggat accgtaattt ttcctcaaaa atcgcatttt gtagaaaatg
     1861 gataataagc gaatgtgtta ttttctgcaa ctttttcaag aaaaatattc aaaaagcgtt
     1921 cacaaatgcc aacaattttc agtttggtgg atacggtggc tatgaaacat cccgaacgtt
     1981 aacagattgg gttgtgagtc gtcaacgtgg atggggaact ccaattccaA TGATCCAAAC
     2041 CAAAGACGGA AAACGAGTTG CTGTTCAATT AAATAAACTT CCAGTCCTTG GGTCAGATCG
     2101 CGGGCAAGAA GTAGATAAAG AAAGATTCGG AGAATCCGGA GTATTCGACA CAGATACTCT
     2161 TGATACATTC TTTGATTCTG CATGgtagag tatatatctg aaatgtacat ttggattaaa
     2221 cttgaatatt tttagGTACT ATCTTCGATA CCTTGACAAC AAAAATAAAA GCGAATTGAT
     2281 TTCCAAAGGA GCAATGAAGA ATATGCCTGT TGATGTCTAT GTTGGAGGAA TTGAACACGC
     2341 AGCGGTGCAC ATGTTTTTTG CTAGATTTGT TGCATATTTC TTGAAGGATA TCAATGTTAT
     2401 CCAGgttggt aaaaactatc atttttaggt ttaatcaaaa tttaacattt tccagACAGC
     2461 TGAACCATTC ACTGACCTCA TCCCACAAGG AATTGTTCGG GGAAAGACGT TCATCGAAAA
     2521 AGAAACAGGA AAATATCTTG GATCAGAAGA TGTTCAACAA TCTGGAGATA AACTCACATT
     2581 GAAGAATGAT TCATCTGTTG AAGTTGACGC TGTCTATGAA AAGATGAGCA AATCGAAAAA
     2641 CAACGGCGTA GATTTGGCTG CAATTTTGGA TTCGGAAGGA GTTGATATGA CAAGACTTAG
     2701 ATTACTTGAA GCTGCAGCTC CGAGAGCTCC CATTAATTGG GGCGAAACAG ATTTAAAGGG
     2761 AATCAAGAAA CTTTTGGATC GAATTGCGGC AATCAATTCG CAAATTGTTG ATGCACGAGA
     2821 TAATGGAAAA CCAATAGAGT TGAAGAAAGA AATTGATGGA AGTATAAAAG AAACATATAA
     2881 CTTTTTCGTA AGAAATGTTG GAATGTGTCT AGAAGTACTT CATCTTCATA ATACTGCGTT
     2941 GATGAGAATA CAGGGATTCA CTAATGCATT AAAGgttatt taacaaatat ttttaaagtc
     3001 tgaaatgatg ttttgatatc actttttaaa gaaaaaaacc acaaactaaa atgatttatt
     3061 tcagAAAGTT GATCCAATCT ACCTGGCTCA CAGCCCAGAA GGCCAACGAG CGGTGAAATC
     3121 GTTGATTATT ATGCTTCAAg tagtttttaa gttctaaagt gcaatctgtt cattaatttt
     3181 ctagGTTTTT TGTCCACACG TCGCTTCCGA AATGTGGTCA GCATTAGCTA ATGATGGTTC
     3241 ACTGGTTTCC GATCAAAAGT GGCCCGAAGT TGATAAAGAT GCTGATATTG AATTTCTTCT
     3301 GATGATTGAT GGTGTTAGTG GTGGTAGGCC TTCTCTTGAT CGGCGGATTA TTGAAGAAAT
     3361 GTCTATGGAT CAGTTATGGG AACGAGCTAC ATTTTATGAA CACGCAGATA TTCTTCGAAT
     3421 GATGAAAGAA GATGGAATGA AGCTGAAAAA TTCAAGTTAT ATTTCTAGGA AAGgttaggt
     3481 tttgtctgtc tagttaccaa ctttagatta caataagaaa tctaagttat taagttatta
     3541 acaaattttc agGTTTCCAC GTAACTCTAT CGTGTAGCTT GGAAGGTGAC AAAGAAGAGA
     3601 ACCGCAAGAA AATTGGCAAA ATTCTGGACA GAATTCAAGC AGAGAAGAGA AAGTTGGAAA
     3661 TCGGGAAGAA AGGTGGAAAG AGGAAGAAAG AAAAGAAGAT TGTGAATAAG TGActtattt
     3721 tactggagaa cattagttta atttttaaca gttttgaaat atatattagc ttagaaatag
     3781 gttgttgttt tattgttttc tgaataattt ttatatttac gggcttgttt attgtttttc
     3841 atgaaaataa atttgttgag tgactccaac ttaccagctt cgaaattgaa tggacgaaac
     3901 gcaattcgcg ttccgtgtct tactacttta cgttgccata acttcgtata gcatacatta
     3961 tacgaagtta ttgcctgaga ccaGCTAGCA AGCTTGCATG CCTGCAGGTC GACTCTAGAG
     4021 GATCCCCGGG TACCGAGCTC aggtctctga gctcaggaaa cggagaatct gtgcggcaat
     4081 gtcttatcat ttgaaacgcg ccgtatcgat gctgacatca tgcggctccc gtgttagtcg
     4141 agaagatggg tacgggcctt tattcatcca acactctacg gcttcttcaa gagctagtag
     4201 ggcaccctgt agttggaaag ggattatttc gtatggcgag cccataccgt ctttcttgcg
     4261 gaagcttaac acgataggaa gttggatagt ttcgaacgat ggttccatac ggaacgctgt
     4321 ctcaaggatg agtctgacgg agtgtaactc gagagcCAGA gtaaggtatt tacgataaaa
     4381 gaatggaaat ttgtcgatta tatacgattt cgtgttttca gtataactaa aaaattgagt
     4441 atataaacca tcgagacaag tctccttgat tactgtaatt ttcaacctca ttactaatac
     4501 gatactatgt attctttgcc aaatagattc gtattcataa aattaaaaaa aaaaacgaaa
     4561 aggttaaaat taatcattca gacaagtaaa taagacattt aatcagttca cataatatca
     4621 ggaaaaatga ggctTCATCC GTAAAGTTGC TCCCAGAAAA ACATTGAACT GAAATAGCAG
     4681 AAAGTACCAC AAATCACAGG CAACGGCAGA GCAGGAGTTG TGGATTCCTc tgaaacgaga
     4741 caattagtat tgaacttcgt gattcatatg gtgattacCA GTTGAAAAGA CAGTGAGAGT
     4801 CACAACAAGT CCAAATAAAA TACCAAGAGC GGCAGAAATG ACTGCAAATG GACATCCGCT
     4861 GGCAGCCGCT TGACCAATCA GAAGACTGTA GAAGACGAAA TCTCCAAAGC CGAGACGAAG
     4921 TACTTctgaa actttctcgt aaaaaaagct ggtaagagag taagcttaat atgacgaaac
     4981 tattattacC TCCATCATTA AGGGCGTCCG CAGCAGTGGC TGGCCGTTTA TCATTGTTCG
     5041 GTAGACTGTT AGAGACCAAA TCATCCCACT CCTTCTGATC ACATTCCTCA GCAGTGCTTA
     5101 TATCAGATGT TGACACAGTG GTGCTTGAGT CGGAAGACGA TAAATTAGAT GATCCACTTG
     5161 AAGTTTCAGA TGTATCACTC TCATCATCAC TGAGCTCCTC GGTACTGTTC TTTTCCTTTA
     5221 ATTCGATTGG TGATGGCTCG GCTTCTActg aaaaagaaaa aatgaataat agcaactgtc
     5281 attcttataa agatctaaat gatttattta attcttacCT AATGGGCTAT GCTCAGTTGG
     5341 TACCGAATCT GGATTGATTG CAGCCCGACG TTGTCTGATC TTTTGATAAA ATTCATCATC
     5401 TTGAGCTTCA CGTTTTGTAT AATATTCAAT CGTTTGCTTC ACGGTTCTTC TGATTGTACT
     5461 CTCCTCTCCT TCATTTGTCT CTTCTTGATT GGATCCTGCA GTTAAACGTT TTTCATTAGC
     5521 AGAAAACATA ATTAAATTGA GAACctgaaa atgaaaataa tcagatttat tttgaaaatt
     5581 gctattctta cGCATTTACT GTAGTCTGAA GCCTTTTCTT GAACTTTTTT GAGTGGACCC
     5641 ATCGGTGCTA AAACGGCAAA GAGATctgga aaaacttaat taaatgcata agagcacagg
     5701 aagaaaacaa acCCCAAAAT AGAACAATCC AGAGAACAAA CCAAGTGGTT TTGCTTGGAA
     5761 AAACTCGTAG GTAGAAAACG GAGATAAGAG AACAGTTTGT GACAACGAAT ATTTGATGAA
     5821 GAATCAAAGA ACTGTTAGAG AAGAATGCct taaaataatt attaattttt atttggttac
     5881 ataacactta cATAGATCCC TCCGAACCCA TATACAACCG TTGGAACCAC TATCAACACA
     5941 ATTGTCATGT AATATTGATT GTTGTCATCT TGGTCAAATA CTTGTGAAAA CATATCATGA
     6001 AGAGTCTGCG CGGATACCCC AAACAATATC AAAAGACATG AAAGTGTGAG CCAGGCTTTT
     6061 ACGATACGAC GGAAATCAAA GAGTACGAAA GCGAGCATTA TGAAAGAGAC GCATCCAAGA
     6121 ACGAGAATTG TACCAACTCC ATTGATctga aatgaaaata tgagaactga aagtggaaga
     6181 gagtattaaa ttattataat gtacgagtgg caaacttttt tgataaacta caaagaaatt
     6241 caatttatgc ttcgtgcgaa acgtagacaa ataggtaggc aagtaggtag gtagaccacc
     6301 ggaagacgtg tcttcctatt tcttgccggt ttttaaaatg ataaaagtat tcaacttctt
     6361 gttgtgaaat gtaatgttgg atgtctcttt tcttgaattg caggatcaat ccgaaataat
     6421 caagctccaa aacatttggg gaatcatgcc cagaaataac tctagtaaat taagaattaa
     6481 aatgttattc tctaactcaa aaactcagaa tgtgcagtcc aaactcacAA ATCCATCCAA
     6541 CAGCAAATTT CCAGTTGTTT GCTCAAACGA AGGATCCAAA AAATAAGTCT TGCTCAATTC
     6601 AGAATTCACT TCCATGTTGT AAACTCCAAT CCAAATAGAC AATGTCAGTG ACATATTGGC
     6661 AATAACAGAG AACAGTGTCC ATCGTAATTG TGAAGATCGC ACTAATTCGC TAGAAATCGA
     6721 TCGAAGGGTG TCCATtttta gacaggtaat aaaattaata ttagcacaag agatttgtat
     6781 gaaattgatg agttggcaga caaaagtatg acacgtgcat gtttgtactt tggtttggat
     6841 cacggggtca cgatgaaatc attttaaaat attattttgg aagaatattg ccgtttctaa
     6901 cattgaagtg aaaaattgaa caagcattgt acatgtcgtg cttttttcta gaaatgctct
     6961 ctttcagagc ttgatcagaa caaccactct acagtactcc aattcgtttc gaaaaaccct
     7021 tcttctagga tcctaagctt ctggtgcgtc ctcttctgga cgcgtcctct tctgggcgcg
     7081 tcctctggag cacaccgagt ttggaaaaat ttaataaaaa tttttaaacc aggagatcac
     7141 gcaaaaaggg ttgaaacata gcgcgctgag attgagcgct agaggatatc aaaagcaacc
     7201 gtgttactct agggtgatcc aaaacgaaat tttgaatagt aaaatatcca aaaattgtct
     7261 tgtattgcaa ctcacattgt aagtaaactc ctgaacaaag aaattttcaa ataaactact
     7321 tgaagtgttt cctttactga catgaatgtt tatacttaaa actttataaa attcaaaaat
     7381 tgaatttgaa aggcacatgg aaaagcaacg gtgaagtata gaaagtagtg attgattgat
     7441 tgtgagaggg ataaaaaggg ataaattttg agacgtttaa cctgaaaaag aaaacaaaac
     7501 gaaagaagag agatgaaaaa aatctcgtca tattcttgac ttttggtcaa agagaaccca
     7561 tttggttttg aaggggaaag aagtgatgaa aaatttcaga attcgaggaa agaaaaaaaa
     7621 aacgacaaaa tgtacaggta ttcgtagagg agatgaaaat tgatcgaatg aagaacaact
     7681 ctattgcttt gaatccaaca aaaaaaggca cttgaatcac tatgttcgat tgatgttttg
     7741 acttgatgct gatgcagcca ttactgtttc tgtctcgtat cggcattccg tttttaggcg
     7801 tacaaagtcc tttgccacct gtaaaaatga aaaatgatta ttgaattttt tttggaaata
     7861 tgtgagtttc taaaaacgct catataatga tttaacagga ccaaatacca taattaaaca
     7921 cttcaaaatt ttcagtcaaa gtttcgacaa actgccaaat tgtggaaaaa ttttttagag
     7981 agggtcttat aaataatttc tttggtcgct ttcctgaatt atgtgaaaac taaaatttca
     8041 gatttttttc tcaagctttt gaa   
//
