LOCUS       1__mosTI_chrIV_8        4006 bp    DNA        linear       24-JUN-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >IV:8617211-8619131
COMMENT     MosTI landing site kstSI37 on Chr. IV with partial unc-119
            selection.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(join(3064..3240,3650..3752,3805..3932))
                     /locus_tag="F41A9.3"
                     /label="F41A9.3"
                     /ApEinfo_label="F41A9.3"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#72fa78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             1943..1999
                     /locus_tag="cbr-unc-119 exons 3/4"
                     /label="cbr-unc-119 exons 3/4"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(353..514,561..672,724..809)
                     /locus_tag="F42A9.8"
                     /label="F42A9.8"
                     /ApEinfo_label="F42A9.8"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#72fa78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             2253..2629
                     /locus_tag="cbr-unc-119 exons 3/4(1)"
                     /label="cbr-unc-119 exons 3/4(1)"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           810..881
                     /locus_tag="3'UTR (wormbase annotation)"
                     /label="3'UTR (wormbase annotation)"
                     /ApEinfo_label="3'UTR (wormbase annotation)"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(2644..2663)
                     /locus_tag="sgRNA insertion"
                     /label="sgRNA insertion"
                     /ApEinfo_label="sgRNA insertion"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           complement(3027..3063)
                     /locus_tag="3' UTR Wormbase annotation"
                     /label="3' UTR Wormbase annotation"
                     /ApEinfo_label="3' UTR Wormbase annotation"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             2098..2209
                     /locus_tag="cbr-unc-119 exons 3/4(2)"
                     /label="cbr-unc-119 exons 3/4(2)"
                     /ApEinfo_label="cbr-unc-119 exons 3/4"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    882..898
                     /locus_tag="LH ChrIII"
                     /label="LH ChrIII"
                     /ApEinfo_label="LH ChrIII"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           882..887
                     /locus_tag="3'UTR"
                     /label="3'UTR"
                     /ApEinfo_label="3'UTR"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    882..898
                     /locus_tag="sgRNA"
                     /label="sgRNA"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     909..942
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     943..946
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        943..946
                     /locus_tag="Punc-119"
                     /label="Punc-119"
                     /ApEinfo_label="Punc-119"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    955..2629
                     /locus_tag="partial cbr-unc-119(+)"
                     /label="partial cbr-unc-119(+)"
                     /ApEinfo_label="partial cbr-unc-119(+)"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2675..2974
                     /locus_tag="Random sequence - no in frame stop and nopolyA
                     consensus"
                     /label="Random sequence - no in frame stop and nopolyA
                     consensus"
                     /ApEinfo_label="Random sequence - no in frame stop and
                     nopolyA consensus"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2983..2985
                     /locus_tag="sgRNA(1)"
                     /label="sgRNA(1)"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2983..3026
                     /locus_tag="RH chrIII"
                     /label="RH chrIII"
                     /ApEinfo_label="RH chrIII"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 tagtaagcta tggaaaataa aagcggtaac tcgttcgagt tgagtctttc tacataattt
       61 acgaattttt tgggattttt gagaaaaatc cttgtttaaa attcaacttt gaagatttac
      121 aataataaaa taatacccga aaaaaacaga aaaataaaaa tgaacaaaaa acaacaaaat
      181 ctgaaaattg aaaaaaatag tgtctagcgg ggcacacttg catacattct gtctgcgtct
      241 cttcgcttca acatactgtc tcgacagtaa ttttcttttt caaagttttt tatttcattt
      301 taattcatta tttccgcttc aaaaagtaat ttgatatttt tttagagcaa aaATGATGTC
      361 ATCGGGACCC TTGGATCCAT CAGATGTCAC GTTATTCACT TCATACCCCA GTCCCATAGT
      421 TGTTCCGCTC GAATTCATAG AAGAACAGTC TAAATCGTCG AGAGAAGAGC TGGAAATTGA
      481 TGAAAGCGGT CTTTATATTA TGTCGAAAGC ACTCgtaaat tggaaaaatg ttgctcgctt
      541 cgttatattc tatttttcag GAAGAATTCA TGCGAACAGT GATGCGAGGA GCAACGCAGA
      601 ATGGTTCCCG ACTTCTAGAC TACGATTCAA TTGCCGAGTT CTTTGCCAAT TCAGAGTTCA
      661 GGGCGCTGAA AGgtaagtcg aattaaaaaa aaattgagaa aataaatacc gagttttttt
      721 cagATTTCTT CCCTGAAAGA GTCCGGTTCG ATGAAATTGA AAGCGAAATC TTACCAACTC
      781 GTGCCACAGG ACCATCTAAC TCTTCATGAc ttatttttta cagtaattct gtgatgcaca
      841 ttttccaaaa agattcacgt ttttttttga ataaacaatc ttaactctca agatcaaatt
      901 acgttgccat aacttcgtat agcatacatt atacgaagtt atttatgcaa gcaactttga
      961 gccaatttat ccaagtcctt gtaaaaagta ttcgaaaatt gttaacggat aaaatgttta
     1021 ttataatatc aaaaacaatt tgtcagttga ccactttttg atatatttga cagaaaccgg
     1081 gatgaattgg ctcaaagtta gggcgccctt ctattacagg gtttctgata acaaaccggt
     1141 tattaactcc caacaaggga tgatttcaat tcatcatgct caaattgacc caaattaagt
     1201 tacatgacaa attcatcgcc cttttcaact cttcttggct catcatctgt tattctgttc
     1261 ctattctgtc tgcaccctat acccctttgc atactctctc gtcattcctt tcttggatag
     1321 tgtcttcttc tccccagctc tgctacttct atgacttgcg cccgctgctt ttccgctcgt
     1381 tcctctcctc tctgacgtct tcttctgctt cttttctcct tcctttttcc cttctcaatc
     1441 ttcctctttt tcccatttct gtcaatcatt cgaagaagaa gaagaagaag accctcattc
     1501 atttcatttt tttttctgtc ggtgtgtgct gctcggttaa gagtgagctc tctattccac
     1561 gtcttcttct ttttcttctt cgattcgaat caatcactcc accaaaacgc attcgttttt
     1621 gggattcacc ccgcggttcg caaTaggttt ctttttcaaa ttattagcgt tataaataga
     1681 aaaatggtgg agtttcaata aaaatgataa tttacaaagt gtattttgat tacatgtact
     1741 caaaaaggtt gaaatttttc ataccagttt tccggaaatc catctgatat cattatcgta
     1801 tttttctttt taaaaatgtt ttcaaaaaaa aacaaaatat agctggttat ttggcaccct
     1861 ctaattacca ttttcctgtc acaccacact cttttccttc ttccacttct ttccgttttc
     1921 agccgcttcc aaccaaaccg atATGAAAGC CGAGCAACAA CGATCGATTC CACCCGGCTC
     1981 GGCGACCTTC CCGTCGCAGg tgagactcag aaaactagag aaaccgctca actaactctt
     2041 gatatccgat ttcattcttt tctcttttct ttttgttgaa cttttccata tttccagATG
     2101 CCACGGCCAC CACCAAGCAC CGAACAAGGA ATCACAACGG AATCGGAGCT TGCGAAGAAA
     2161 GCTCAAATCA CTCCGAACGA CGTTTTAGCA CTTCCGGGAA TCACTCAAGg tatcttgctc
     2221 cctttggatt tcagtttaaa acataaattt agGATTCTTA TGCTCCCCAT CTGCGAACAT
     2281 CTATAACATC GAGTTCACCA AGTTCCAAAT CCGTGATCTG GACACTGAGC AAGTGCTTTT
     2341 CGAGATCGCC AAACCGGAGA ACGATCAGGA GAATGATGAG TCGCCACAGG AGTCGGCAAG
     2401 ATACGTGCGT TATAGATTTG CTCCAAACTT TTTGAAACTC AAAACGGTCG GAGCAACTGT
     2461 GGAATTCAAA GTAGGAGACA TCCCAATCCA TCATTTCCGA ATGATCGAAC GTCACTTCTT
     2521 CAAAGATCGC CTTCTGAAGT GTTTTGACTT TGAATTCGGA TTCTGTATTC CGAATTCACG
     2581 AAACAACTGT GAACATATCT ATGAGTTCCC TCAACTCTCT CAACAACTCT GCCgGAGACG
     2641 ccaGTGCATA ATCAACCTTC CCCCGTCTCc GCAAtcagga aacggagaat ctgtgcggca
     2701 atgtcttatc atttgaaacg cgccgtatcg atgctgacat catgcggctc ccgtgttagt
     2761 cgagaagatg ggtacgggcc tttattcatc caacactcta cggcttcttc aagagctagt
     2821 agggcaccct gtagttggaa agggattatt tcgtatggcg agcccatacc gtctttcttg
     2881 cggaagctta acacgatagg aagttggata gtttcgaacg atggttccat acggaacgct
     2941 gtctcaagga tgagtctgac ggagtgtaac tcgagagcCA GAacctggac ccgtaacatt
     3001 ttaagccagt atttaaaaga acaaaataca aacaatcatt tattcgagtt taataagtaa
     3061 tcaTTATCCC GATGACGATG CATTCTGGAC AGTCGAGGGT ATACCAAACA TTTGTACAAT
     3121 GTCAGGAATT TCCAAAAAGT AGACTTTATC ACGCATAGAT GCTACACCGA AAAAGAAGAA
     3181 TATTCCAGAG AAAAGAATAG CTCCAACGAC TCCACCCAAT CCACACATGA AATCGTGTAC
     3241 ctagaaaaac taattatttt tgtatattga gacttcaaat ataagctctt aaaacattac
     3301 tgtagctata ttatggttat tatcgaccag tttttagatt ttacgaaaat acaggcaaaa
     3361 ttacagtaca gcaatgcgac tctcgccacg aatgaaatgt tttaaaatgt agttcggaaa
     3421 aggtgtgcgc ctttacacag taacccaact ttagtagttt cgcaatttag aacattctat
     3481 ctcaaaaaca actttcgttg aaaaaataaa cgaaaaagaa acattccgaa ataacgaaga
     3541 attggttact gtatcaatta aaggcgcaaa agtttgttcg tggcgagacc gggtaccgta
     3601 attttgggcg aaaacaccat aaatgcgtca taatattttt ccttcgtacC ATTTTATTTT
     3661 CAAGTGTTTC TGAACTTCTA TTGGTTCCTT GCTGGGCATC AGCCACAATC TTCGGTGCTT
     3721 TGACAGCACC ATATGTCGAA GCTTGAAACA TTctgaaaat gtattagtga gggttagtca
     3781 agtggattca tcagtcaaac atacTGTGTT GTGACCTCAC TAGTTTCACC AACTTTACCA
     3841 CCAGTTGCGG TATTTTGGGC AGAAACACCC GGGCCTGAAA TACCCAGTGC TGATGGTCCT
     3901 GCAGCTTTAT TGCTCGCAGA TGATGTTGCC ATttttgaaa tgatcggaat tgaggaaaaa
     3961 tcggaaatga ttgaaattca tttgatgaat agaacagtgg attttc 
//
