LOCUS       New_DNA                 1921 bp    DNA        linear       24-JUN-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >IV:8617211-8619131
COMMENT     MosTI landing site, Chr. IV. Genomic region before insertion of
            landing site. 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1022..1040)
                     /locus_tag="sgRNA for insertion"
                     /label="sgRNA for insertion"
                     /ApEinfo_label="sgRNA for insertion"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             join(75..202,255..357,767..943)
                     /locus_tag="F41A9.3"
                     /label="F41A9.3"
                     /ApEinfo_label="F41A9.3"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#72fa78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     3'UTR           944..980
                     /locus_tag="3' UTR Wormbase annotation"
                     /label="3' UTR Wormbase annotation"
                     /ApEinfo_label="3' UTR Wormbase annotation"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(join(1113..1198,1250..1361,1408..1569))
                     /locus_tag="F42A9.8"
                     /label="F42A9.8"
                     /ApEinfo_label="F42A9.8"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#72fa78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     3'UTR           complement(1034..1112)
                     /locus_tag="3'UTR (wormbase annotation)"
                     /label="3'UTR (wormbase annotation)"
                     /ApEinfo_label="3'UTR (wormbase annotation)"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 gaaaatccac tgttctattc atcaaatgaa tttcaatcat ttccgatttt tcctcaattc
       61 cgatcatttc aaaaATGGCA ACATCATCTG CGAGCAATAA AGCTGCAGGA CCATCAGCAC
      121 TGGGTATTTC AGGCCCGGGT GTTTCTGCCC AAAATACCGC AACTGGTGGT AAAGTTGGTG
      181 AAACTAGTGA GGTCACAACA CAgtatgttt gactgatgaa tccacttgac taaccctcac
      241 taatacattt tcagAATGTT TCAAGCTTCG ACATATGGTG CTGTCAAAGC ACCGAAGATT
      301 GTGGCTGATG CCCAGCAAGG AACCAATAGA AGTTCAGAAA CACTTGAAAA TAAAATGgta
      361 cgaaggaaaa atattatgac gcatttatgg tgttttcgcc caaaattacg gtacccggtc
      421 tcgccacgaa caaacttttg cgcctttaat tgatacagta accaattctt cgttatttcg
      481 gaatgtttct ttttcgttta ttttttcaac gaaagttgtt tttgagatag aatgttctaa
      541 attgcgaaac tactaaagtt gggttactgt gtaaaggcgc acaccttttc cgaactacat
      601 tttaaaacat ttcattcgtg gcgagagtcg cattgctgta ctgtaatttt gcctgtattt
      661 tcgtaaaatc taaaaactgg tcgataataa ccataatata gctacagtaa tgttttaaga
      721 gcttatattt gaagtctcaa tatacaaaaa taattagttt ttctagGTAC ACGATTTCAT
      781 GTGTGGATTG GGTGGAGTCG TTGGAGCTAT TCTTTTCTCT GGAATATTCT TCTTTTTCGG
      841 TGTAGCATCT ATGCGTGATA AAGTCTACTT TTTGGAAATT CCTGACATTG TACAAATGTT
      901 TGGTATACCC TCGACTGTCC AGAATGCATC GTCATCGGGA TAAtgattac ttattaaact
      961 cgaataaatg attgtttgta ttttgttctt ttaaatactg gcttaaaatg ttacgggtcc
     1021 aggttttgat cttgagagtt agattgttta ttcaaaaaaa aacgtgaatc tttttggaaa
     1081 atgtgcatca cagaattact gtaaaaaata agTCATGAAG AGTTAGATGG TCCTGTGGCA
     1141 CGAGTTGGTA AGATTTCGCT TTCAATTTCA TCGAACCGGA CTCTTTCAGG GAAGAAATct
     1201 gaaaaaaact cggtatttat tttctcaatt tttttttaat tcgacttacC TTTCAGCGCC
     1261 CTGAACTCTG AATTGGCAAA GAACTCGGCA ATTGAATCGT AGTCTAGAAG TCGGGAACCA
     1321 TTCTGCGTTG CTCCTCGCAT CACTGTTCGC ATGAATTCTT Cctgaaaaat agaatataac
     1381 gaagcgagca acatttttcc aatttacGAG TGCTTTCGAC ATAATATAAA GACCGCTTTC
     1441 ATCAATTTCC AGCTCTTCTC TCGACGATTT AGACTGTTCT TCTATGAATT CGAGCGGAAC
     1501 AACTATGGGA CTGGGGTATG AAGTGAATAA CGTGACATCT GATGGATCCA AGGGTCCCGA
     1561 TGACATCATt tttgctctaa aaaaatatca aattactttt tgaagcggaa ataatgaatt
     1621 aaaatgaaat aaaaaacttt gaaaaagaaa attactgtcg agacagtatg ttgaagcgaa
     1681 gagacgcaga cagaatgtat gcaagtgtgc cccgctagac actatttttt tcaattttca
     1741 gattttgttg ttttttgttc atttttattt ttctgttttt ttcgggtatt attttattat
     1801 tgtaaatctt caaagttgaa ttttaaacaa ggatttttct caaaaatccc aaaaaattcg
     1861 taaattatgt agaaagactc aactcgaacg agttaccgct tttattttcc atagcttact
     1921 a     
//
