LOCUS pCFJ1655___pDEST 7383 bp ds-DNA circular 19-DEC-2013 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Generated by Gibson Assembly. Grow in ccdB competent cells. Select with Cm and Amp. COMMENT COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers misc_recomb 37..160 /label=attR4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(1616..1739) /label=attR3 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(204..413) /label=ccdB /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 7155..7376 /label=Mos1 drosophila /ApEinfo_fwdcolor=#7b1bff /ApEinfo_revcolor=#7b1bff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 7127..7154 /label=Artificial 3'ITR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4469..4765 /label=Mos1 drosophila(1) /ApEinfo_label=Mos1 drosophila /ApEinfo_fwdcolor=#7b1bff /ApEinfo_revcolor=#7b1bff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 6311..6993 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 4849..4917 /label=LacZ alpha /ApEinfo_fwdcolor=#6495ed /ApEinfo_revcolor=#6495ed /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 5554..6213 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(851..1507) /label=CmR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind complement(1747..1763) /label=M13F(-20) /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 6..21 /label=M13R(-24) /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 1773..2523 /label=Prps-0 /ApEinfo_fwdcolor=#ff101a /ApEinfo_revcolor=#ff101a /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 3595..3645 /label=SynIVS.S /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 2524..3651 /label=hygR CeOpt /ApEinfo_fwdcolor=#1227ff /ApEinfo_revcolor=#1227ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 3652..4453 /label=unc-54 3'UTR /ApEinfo_fwdcolor=#ff0118 /ApEinfo_revcolor=#ff0118 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 2701..2751 /label=SynIVS.R /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 intron 3728..3778 /label=SynIVS.L /ApEinfo_fwdcolor=#c5ff39 /ApEinfo_revcolor=#c5ff39 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac 61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga 121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg 181 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg 241 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg 301 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt 361 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc 421 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt 481 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc 541 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg 601 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatatt 661 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc 721 tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta tatattctta 781 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc 841 ctctagatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc 901 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca 961 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca 1021 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa 1081 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat 1141 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg 1201 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca 1261 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa 1321 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg 1381 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct 1441 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt 1501 tctccatttt agcttcctta gctcctgaaa atctcgacgg atcctaactc aaaatccaca 1561 cattatacga gccggaagca taaagtgtaa agcctggggt gcctaatgcg gccgccatag 1621 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt 1681 taatatattg atatttatat cattttacgt ttctcgttca actttattat acatagttga 1741 taattcactg gccgtcgttt tacaggccgg atATGAAGAT GAACGCAAGG AGCCTGATGC 1801 AGCGACTCTT GCTAGGAGAT CATCAGAAAG CTTATATTTG ATTAGTGACG TCGCATCAAA 1861 GCTGAACACA TAAATTCTTT AATATTTTAT ATCCCCATAT TATGCACCTG AGAGGAATGA 1921 AGAAATTTGC TGATACTCCC GGTGAATTCA TGTCTTGATG GTTGTGCGCA TTGTGGTAAT 1981 ATCTGAAAAA TTTCGAAGTC AGTTTTCTGA TAAGGCAACA ACTAATAAAT TGAGAATCAA 2041 TTTACCCTTA AGGAACGATG TTCGCTTCTG AATTTATTAA TAAATTTTTC CGTAATTCAA 2101 TTCAAAATTG TCATATTTAA GAAAAAAACA TCAAAAATGA CAAATTCAAT ATTTTAATTA 2161 ATTTTTGCTT TCGTCGTAAA TCTACACACG CGTCTCTTCC GTGCGAGAGT CCAAGCCAGC 2221 AGCCAAATTC GTTGACTGAG TATTCAACGT TTATACGTTG TCGGCAACGA GAAATAGGAA 2281 AATGCATCGG GAAATGTTCT TTTTTCGATT TTTTCCAAGG TTTTGACAAA TTTTACCACG 2341 AATTTTGCTA TGTTTTCAAT TAAAAAATAT GTTATTCAAC TGTTTCTATG AGGAAAATAA 2401 GGCTTTGCAT GTAATTTTCT TATTCAGCAT AATTTTTAAT TAATTTGAAT TTTCTGTCCT 2461 AACGTTTATT TTGTTTTCTT GGTTATGACT GATCTGAAAT TAATTTTTGA ATTTTAAGGT 2521 AATATGAAAA AACCCGAGTT GACCGCCACA TCCGTAGAGA AGTTCCTCAT CGAGAAGTTC 2581 GACTCCGTCT CCGACCTCAT GCAACTCTCC GAGGGAGAGG AGTCCCGTGC CTTCTCCTTC 2641 GACGTCGGAG GACGTGGATA CGTCCTCCGT GTCAACTCCT GCGCCGACGG ATTCTACAAG 2701 GTAAGTTTAA ACATATATAT ACTAACTAAC CCTGATTATT TAAATTTTCA GGACCGTTAC 2761 GTCTACCGTC ACTTCGCCTC CGCCGCCCTC CCAATCCCAG AGGTCCTCGA CATCGGAGAG 2821 TTCTCCGAGT CCCTCACCTA CTGCATCTCC CGTCGTGCCC AAGGAGTCAC CCTCCAAGAC 2881 CTCCCAGAGA CCGAGCTCCC AGCCGTCCTC CAACCAGTCG CCGAGGCCAT GGACGCCATC 2941 GCCGCCGCCG ACCTCTCCCA AACCTCCGGA TTCGGACCAT TCGGACCACA AGGAATCGGA 3001 CAATACACCA CCTGGCGTGA CTTCATCTGC GCCATCGCCG ACCCACACGT CTACCACTGG 3061 CAAACCGTCA TGGACGACAC CGTCTCCGCC TCCGTCGCCC AAGCCCTCGA CGAGCTCATG 3121 CTCTGGGCCG AGGACTGCCC AGAGGTCCGT CACCTCGTCC ACGCCGACTT CGGATCCAAC 3181 AACGTCCTCA CCGACAACGG ACGTATCACC GCCGTCATCG ACTGGTCCGA GGCCATGTTC 3241 GGAGACTCCC AATACGAGGT CGCCAACATC TTCTTCTGGC GTCCATGGCT CGCCTGCATG 3301 GAGCAACAAA CCCGTTACTT CGAGCGTCGT CACCCAGAGC TCGCCGGATC CCCACGTCTC 3361 CGTGCCTACA TGCTCCGTAT CGGACTCGAC CAACTCTACC AATCCCTCGT CGACGGAAAC 3421 TTCGACGACG CCGCCTGGGC CCAAGGACGT TGCGACGCCA TCGTCCGTTC CGGAGCCGGA 3481 ACCGTCGGAC GTACCCAAAT CGCCCGTCGT TCCGCCGCCG TCTGGACCGA CGGATGCGTC 3541 GAGGTCCTCG CCGACTCCGG AAACCGTCGT CCATCCACCC GTCCACGTGC CAAGGTAAGT 3601 TTAAACAGTT CGGTACTAAC TAACCATACA TATTTAAATT TTCAGGAGTA AAATTCCAAC 3661 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 3721 CATCAGAGTA AGTTTAAACT GAGTTCTACT AACTAACGAG TAATATTTAA ATTTTCAGCA 3781 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 3841 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 3901 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 3961 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 4021 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4081 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4141 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4201 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4261 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4321 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4381 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4441 TAGTGTCTAT GGGAGCTTGA TccggatcTG CGGCTTACTC ACCAGACCTG GCCCCATCCG 4501 ATTACCACCT ATTCGCTTCG ATGGGACACG CACTCGCTGA GCAGCGCTTC GATTCTTACG 4561 AAAGTGTGAA AAAATGGCTC GATGAATGGT TCGCCGCAAA AGACGATGAG TTCTACTGGC 4621 GTGGAATCCA CAAATTGCCC GAGAGATGGG AAAAATGTGT AGCTAGCGAC GGCAAATACT 4681 TTGAATAAAT GATTTTTTCT TTTTCCACAA AATTTAACGT GTTTTTTGAT TTAAAAAAAA 4741 CGACATTTCA TACTTGTACA CCTGATATCT AGATACCTAG GTGAGCTCTG GTACCCccct 4801 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 4861 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 4921 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 4981 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 5041 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 5101 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 5161 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 5221 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 5281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 5341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 5401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 5461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 5521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 5581 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 5641 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 5701 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 5761 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 5821 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 5881 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 5941 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 6001 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 6061 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 6121 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 6181 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 6241 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 6301 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 6361 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 6421 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 6481 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 6541 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6601 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6661 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 6721 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6781 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6841 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6901 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6961 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg 7021 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 7081 gcctttgagt gagctgatac cGCACCGTAC GTCTCGAGGA ATTCTATCAG GTGTACAAGT 7141 ATGAAATGTC GTTTCGAACA TATAGATGTC TCGCAAACGT AAATATTTAT CGATTGTCAT 7201 AAAACTTTGA CCTTGTGAAG TGTCAACCTT GACTGTCGAA CCACCATAGT TTGGCGCGAA 7261 TTGAGCGTCA TAATTGTTTA CTCTCAGTGC AGTCAACATG TCGAGTTTCG TGCCGAATAA 7321 AGAGCAAACG CGGACAGTAT TAATTTTCTG TTTTCATTTG AAGAAAACAG CTGCGGccgg 7381 atc //