{"id":599,"date":"2013-07-30T02:49:49","date_gmt":"2013-07-30T02:49:49","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=599"},"modified":"2014-04-22T08:00:55","modified_gmt":"2014-04-22T14:00:55","slug":"plasmids-2","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=599","title":{"rendered":"Plasmids"},"content":{"rendered":"

miniMos<\/h3>\n

The miniMos reagents are described in: \u201cFr\u00f8kj\u00e6r-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovski A, Moerman DG and Jorgensen EM. Random and targeted transgene insertion in\u00a0C. elegans<\/em>\u00a0using a modified Mos1 transposon.\u00a0Nature Methods<\/em>. (In press)\u201d.<\/p>\n

The plasmids can be ordered either individually (see links below) or as a kit (29 plasmids – 8 co-injection vectors, 16 cloning vectors, 3 positive controls and 2 LacO vectors) from Addgene. Please see this page<\/a> for information about ordering the kit. If you plan to order more than 7 plasmids it is cheaper to order the full kit. Addgene is a non-profit plasmid repository and we do not receive any compensation or commission – it is just the easiest way to distribute plasmids and saves us a lot of time dealing with shipping and MTAs.<\/p>\n

Co-injection markers<\/h5>\n\n\n\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Plasmid<\/td>Description<\/td>Transgene<\/td>ApE file<\/td>\"Addgene<\/td>\n<\/tr>\n
Mos1 transposase<\/strong><\/td>\n<\/tr>\n
pCFJ601<\/td>Induces Mos1 transposition by injection.<\/td>Peft-3<\/em>:Mos1 transposase<\/td>pCFJ601<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pJL44<\/td>Induces Mos1 transposition by heat-shock.<\/td>Phsp-16.48<\/em>:Mos1 transposase<\/td>pJL44<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Negative selection<\/strong><\/td>\n<\/tr>\n
pMA122<\/td>Negative selection, heat-shock inducible.<\/td>Phsp-16.41<\/em>:peel-1<\/em>:tbb-2<\/em> UTR<\/td>pMA122<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Fluorescent markers<\/strong><\/td>\n<\/tr>\n
pGH8<\/td>Visual extra-chromosomal array marker. Red, nervous system, cytosolic.<\/td>Prab-3<\/em>:mCherry:unc-54<\/em> UTR<\/td>pGH8<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ90<\/td>Visual extra-chromosomal array marker. Red, pharynx, cytosolic. <\/td>Pmyo-2<\/em>:mCherry:unc-54<\/em> UTR<\/td>pCFJ90<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ104<\/td>Visual extra-chromosomal array marker. Red, body wall muscle, cytosolic.<\/td>Pmyo-3<\/em>:mCherry:unc-54<\/em> UTR<\/td>pCFJ104<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ420<\/td>Visual extra-chromosomal array marker. Green, ubiquitous, nuclear. <\/td>Peft-3<\/em>:GFP:H2B:tbb-2<\/em> UTR<\/td>pCFJ420<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ421<\/td>Visual extra-chromosomal array marker. Green, pharynx, nuclear.<\/td>Pmyo-2<\/em>:GFP:H2B:tbb-2<\/em> UTR<\/td>pCFJ421<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n
Top<\/a><\/div><\/h5>\n
miniMos cloning vectors<\/h5>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Plasmid<\/strong><\/th>Selection<\/strong><\/th>ApE file<\/strong> (Right click and \"save as..\")<\/th>\"Addgene<\/a><\/th>\n<\/tr>\n<\/thead>\n
Three-fragment Gateway vectors (R4-R3<\/strong><\/td>\n<\/tr>\n
pCFJ906<\/td>cb-unc-119<\/em><\/td>B10 - pCFJ906 - Minimal Mos1, unc-119, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ907<\/td>NeoR<\/td>B11 - pCFJ907 - pDESTR4-R3-miniMos(NeoR)<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ908<\/td>PuroR<\/td>B12 - pCFJ908 - Minimal Mos1, PuroR, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1655<\/td>HygroR<\/td>pCFJ1655 - pDESTR4-R3-miniMos(HygroR)<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1258<\/td>No selection<\/td>C4 - pCFJ1258 - Minimal Mos1, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Multiple cloning site vectors<\/strong><\/td>\n<\/tr>\n
pCFJ909<\/td>cb-unc-119<\/em><\/td>A1 - pCFJ909 - Minimal Mos1, unc-119, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ910<\/td>NeoR<\/td>A2 - pCFJ910 - Minimal Mos1, NeoR, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1662<\/td>HygroR<\/td>pCFJ1662 - pEXPminiMos(hygroR)[MCS]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1272<\/td>No selection<\/td>A6- pCFJ1272 - Minimal Mos1, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Three-fragment Gateway vectors (R4-R3) + hs:peel-1<\/em><\/strong><\/td>\n<\/tr>\n
pCFJ1001<\/td>cb-unc-119<\/em><\/td>C2 - pCFJ1001 - Minimal Mos1, unc-119, peel-1, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1002<\/td>NeoR<\/td>C3 - pCFJ1002 - Minimal Mos1, NeoR, peel-1, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1000<\/td>PuroR<\/td>C1 - pCFJ1000 - Minimal Mos1, PuroR, peel-1, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1656<\/td>HygroR<\/td>pCFJ1656 - pDESTR4-R3-miniMos(HygroR, peel-1)<\/a><\/td>Request Plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1259<\/td>No selection<\/td>C5 - pCFJ1259 - Minimal Mos1, peel-1, [4-3] Gateway<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Multiple cloning site vectors + hs:peel-1<\/em><\/strong><\/td>\n<\/tr>\n
pCFJ1201<\/td>cb-unc-119<\/em><\/td>A3 - pCFJ1201 - Minimal Mos1, unc-119, peel-1, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1202<\/td>NeoR<\/td>A4 - pCFJ1202 - Minimal Mos1, NeoR, peel-1, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1663<\/td>HygroR<\/td>pCFJ1663 - pEXPminiMos(hygroR, peel-1)MCS]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1200<\/td>PuroR<\/td>A5 - pCFJ1200 - Minimal Mos1, PuroR, peel-1, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1273<\/td>No selection<\/td>A7 - pCFJ1273 - Minimal Mos1, peel-1, MCS<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Fosmid cassette<\/strong><\/td>\n<\/tr>\n
pCFJ496<\/td>cb-unc-119<\/em><\/td>A8 - pCFJ496 - 500bp Mos1, unc-119, fosmid cassette<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n
Top<\/a><\/div><\/h5>\n
Positive control vectors + LacO<\/h5>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Plasmid<\/strong><\/th>Transgene<\/strong><\/th>Selection<\/strong><\/th>ApE file<\/strong> (Right click and \"save as..\")<\/th>\"Addgene<\/a><\/th>\n<\/tr>\n<\/thead>\n
Positive controls<\/strong><\/td>\n<\/tr>\n
pCFJ914<\/td>Peft-3<\/em>:GFP:H2B:tbb-2<\/em>UTR<\/td>NeoR<\/td>A9 - pCFJ914 - Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1208<\/td>Peft-3<\/em>:tdTomato(NLS):tbb-2<\/em> UTR<\/td>cb-unc-119<\/em><\/td>A10 - pCFJ1208 - Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1209<\/td>Peft-3<\/em>:tdTomato(NLS):tbb-2<\/em> UTR<\/td>NeoR<\/td>A11 - pCFJ1209 - Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
256x LacO plasmids<\/strong><\/td>\n<\/tr>\n
pCFJ1324<\/td>256x lacO<\/em><\/td>cb-unc-119<\/em> + NeoR<\/td>A12 - pCFJ1324 - Minimal Mos1 [256x LacO, unc-119, NeoR, peel-1]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1319<\/td>256x lacO<\/em><\/td>cb-unc-119<\/em> + PuroR<\/td>B1 - pCFJ1319 - Minimal Mos1 [256x LacO, unc-119, PuroR, peel-1]<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n
Top<\/a><\/div><\/h5>\n","protected":false},"excerpt":{"rendered":"

miniMos The miniMos reagents are described in: \u201cFr\u00f8kj\u00e6r-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte …<\/p>\n

Plasmids<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":394,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-599","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-9F","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/599","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=599"}],"version-history":[{"count":9,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/599\/revisions"}],"predecessor-version":[{"id":875,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/599\/revisions\/875"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/394"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=599"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}