{"id":529,"date":"2013-06-21T14:54:43","date_gmt":"2013-06-21T14:54:43","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=529"},"modified":"2014-03-02T09:19:28","modified_gmt":"2014-03-02T15:19:28","slug":"about-minimos","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=529","title":{"rendered":"About miniMos"},"content":{"rendered":"<h4>About the miniMos transposon<\/h4>\n<p>The miniMos transposon is a method to carry transgenes into the\u00a0<em>C. elegans<\/em>\u00a0genome. \u00a0The method has the advantages:<\/p>\n<ul>\n<li>The insertion frequency and fidelity is high<\/li>\n<li>The exact insertion site can be determined<\/li>\n<li>Large transgenes can be inserted<\/li>\n<li>The miniMos element is active in\u00a0<em>C. elegans<\/em>,\u00a0<em>C. briggsae<\/em>\u00a0and natural isolates.<\/li>\n<li>Transgenes are expressed in the germline at high frequency.<\/li>\n<\/ul>\n<p>The method works by inserting a transgene into a modified Mos1 element (miniMos). The miniMos transposon can carry the inserted transgene (together with a selection marker) into the genome. Insertions are generated directly by injecting worms and take approximately 1 week to generate. The insertion site is random and can be identified by inverse PCR on the strain.<\/p>\n<p>We have released the reagents prior to publication so that other researchers can use them without having to wait.\u00a0Please cite \u201cFr\u00f8kj\u00e6r-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovski A, Moerman DG and Jorgensen EM. Random and targeted transgene insertion in\u00a0<em>C. elegans<\/em>\u00a0using a modified Mos1 transposon.\u00a0<em>Nature Methods<\/em>. (In press)\u201d\u00a0\u00a0if you manage to use the reagents in a publication before we publish the method.<\/p>\n<p>The plasmids can be ordered either individually (see <a title=\"Plasmids\" href=\"http:\/\/wormbuilder.org\/old\/minimos\/plasmids-2\/\">plasmids<\/a>) or as a kit (29 plasmids \u2013 8 co-injection vectors, 16 cloning vectors, 3 positive controls and 2 LacO vectors) from Addgene. Please see <a title=\"this page\" href=\"http:\/\/www.addgene.org\/minimos\/\" target=\"_blank\">this page<\/a> for information about ordering the kit. If you plan to order more than 7 plasmids it is cheaper to order the full kit. Addgene is a non-profit plasmid repository and we do not receive any compensation or commission \u2013 it is just the easiest way to distribute plasmids and saves us a lot of time dealing with shipping and MTAs.<\/p>\n<p>Also, several people in the lab are in the process of developing a gene-trap system based on the miniMos transposon. Please don\u2019t use the miniMos reagents prior to publication to compete with these efforts. We will release the gene-trap reagents as soon as possible.<\/p>\n<h6>Requirements<\/h6>\n<ul>\n<li>The transgene cloned into the appropriate miniMos vector (different selection markers)<\/li>\n<\/ul>\n<h6>Schematic of method<\/h6>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" alt=\"miniMOS\" src=\"http:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/miniMOS.png\" width=\"627\" height=\"687\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>About the miniMos transposon The miniMos transposon is a method to carry transgenes into the\u00a0C. elegans\u00a0genome. &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"more-link\" href=\"https:\/\/wormbuilder.org\/old\/?page_id=529\"> <span class=\"screen-reader-text\">About miniMos<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":394,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-529","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-8x","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=529"}],"version-history":[{"count":4,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/529\/revisions"}],"predecessor-version":[{"id":854,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/529\/revisions\/854"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/394"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}