{"id":523,"date":"2013-06-21T14:50:14","date_gmt":"2013-06-21T14:50:14","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=523"},"modified":"2013-06-21T14:50:14","modified_gmt":"2013-06-21T14:50:14","slug":"about-mossci","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=523","title":{"rendered":"About MosSCI"},"content":{"rendered":"<h4>About mos1-mediated Single Copy Insertion (mosSCI)<\/h4>\n<p>mosSCI is a method to insert a single copy of a transgene into a well-defined location in the\u00a0<em>C. elegans<\/em>\u00a0genome. The method has the advantages:<\/p>\n<ul>\n<li>Transgenes can be expressed at levels close to endogenous gene expression<\/li>\n<li>Transgene insertions are stable<\/li>\n<li>Transgene expression is possible in the germline.<\/li>\n<\/ul>\n<p>The method works by breaking a chromosome at a particular location by excising a Mos1 transposon. The excision creates a double-strand DNA break that is repaired by the cell. In the presence of a DNA template with homology to the breakpoint then the repair process will incorporate DNA from the repair template into the genome. We have incorporated a positive selection marker to select for this event (typically\u00a0<em>unc-119<\/em>) and fluorescent markers to identify extra-chromosomal arrays.<\/p>\n<h6>Requirements<\/h6>\n<ul>\n<li>A transgene cloned into the appropriate targeting vector.<\/li>\n<li>A strain containing a Mos1 transposon at the site corresponding to the targeting vector.<\/li>\n<\/ul>\n<h6>Schematic of method<\/h6>\n<p><img loading=\"lazy\" decoding=\"async\" alt=\"MosSCI\" src=\"http:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/02\/MosSCI1.png\" width=\"627\" height=\"734\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>About mos1-mediated Single Copy Insertion (mosSCI) mosSCI is a method to insert a single copy of &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"more-link\" href=\"https:\/\/wormbuilder.org\/old\/?page_id=523\"> <span class=\"screen-reader-text\">About MosSCI<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":15,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-523","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-8r","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/523","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=523"}],"version-history":[{"count":1,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/523\/revisions"}],"predecessor-version":[{"id":524,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/523\/revisions\/524"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/15"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}