{"id":401,"date":"2013-06-19T23:23:17","date_gmt":"2013-06-19T23:23:17","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=401"},"modified":"2013-06-21T15:08:53","modified_gmt":"2013-06-21T15:08:53","slug":"universal-mossci-insertion-strains","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=401","title":{"rendered":"Universal MosSCI insertion strains"},"content":{"rendered":"
Universal insertion sites\"Uni_sites\"<\/h6>\n

All universal MosSCI insertion sites are targeted by vectors for the ttTi5605<\/em> locus. These vectors include pCFJ150 and pCFJ350.<\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Insertion site<\/th>Locus<\/strong><\/th>Co-insertion marker<\/strong><\/th>Genetic position<\/strong><\/th>Genomic position (WS190)<\/strong><\/th>Genomic environment<\/strong><\/th>Strain<\/strong><\/th>Germline expression<\/strong><\/th>\n<\/tr>\n<\/thead>\n
unc-119<\/strong><\/em><\/td>\n<\/tr>\n
UniI_I ( Neo)<\/td>oxTi185<\/em><\/td>NeoR + unc-18<\/em>(+)<\/td>I:1.17<\/td>I: 6,503,678<\/td>Intergenic<\/td>EG8078<\/a><\/td>Yes<\/td>\n<\/tr>\n
Uni_II (Neo)<\/td>oxTI179<\/em><\/td>NeoR + unc-18<\/em>(+)<\/td>II:1.73<\/td>II: 9,833,502<\/td>In ZK938.3<\/a><\/td>EG8079<\/a><\/td>Yes<\/td>\n<\/tr>\n
Uni_III (Neo)<\/td>oxTi444<\/em><\/td>NeoR + unc-18<\/em>(+)<\/td>III:-0.85<\/td>III: 7,014,336<\/td>In lgc-38<\/em><\/a><\/td>EG8080<\/a><\/td>Yes<\/td>\n<\/tr>\n
UniI_IV (Neo)<\/td>oxTi177<\/em><\/td>NeoR + unc-18<\/em>(+)<\/td>IV:7.43<\/td>IV: 13,048,924<\/td>In scl-10<\/em><\/a><\/td>EG8081<\/a><\/td>Yes<\/td>\n<\/tr>\n
Uni_V (Neo)<\/td>oxTi365<\/em><\/td>NeoR + unc-18<\/em>(+)<\/td>V:1.52<\/td>V: 8,643,273<\/td>In asp-13<\/em><\/a><\/td>EG8082<\/a><\/td>Yes<\/td>\n<\/tr>\n
Uni_V (GFP)<\/td>oxTi354<\/em><\/td>Pmyo-2<\/em>:GFP:H2B + unc-18<\/em>(+)<\/td>V:5.59<\/td>V: 13,783,531<\/td>In F53C11.3<\/em><\/a><\/td>EG8083<\/a><\/td>Yes<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

All strains have been outcrossed 5x. You can follow insertions based on resistance to the drug G418 \u00a0(NeoR) or the fluorophore adjacent to the insertion site.<\/p>\n

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Universal insertion sites All universal MosSCI insertion sites are targeted by vectors for the ttTi5605 locus. …<\/p>\n

Universal MosSCI insertion strains<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":397,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-401","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-6t","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/401","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=401"}],"version-history":[{"count":6,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/401\/revisions"}],"predecessor-version":[{"id":545,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/401\/revisions\/545"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/397"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}