{"id":397,"date":"2013-06-19T23:20:51","date_gmt":"2013-06-19T23:20:51","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=397"},"modified":"2013-06-21T14:29:27","modified_gmt":"2013-06-21T14:29:27","slug":"universal-mossci-sites","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=397","title":{"rendered":"Universal MosSCI"},"content":{"rendered":"<h4>About universal MosSCI sites<\/h4>\n<p>The universal MosSCI sites are insertion sites that allow insertion of the same transgene at several well-defined locations in the <em>C. elegans<\/em> genome.\u00a0The method has the advantages:<\/p>\n<ul>\n<li>One targeting vector is compatible with multiple insertion sites<\/li>\n<li>The local genomic environment is the same for all insertions<\/li>\n<li>Insertions can be followed by the adjacent marker (NeoR or GFP signal)<\/li>\n<\/ul>\n<p>The method works by inserting a transgene into a well-defined landing site at specific locations in the genome. The insertion sites were engineered into the genome and are therefore all identical (except for variations in the co-insertion marker, NeoR or P<em>myo-2<\/em>:GFP:H2B). The insertion sites contain a Mos1 transposon that can be excised. After excision, a repair process copies the transgene from a targeting vector into the genomic location.<\/p>\n<h6>Requirements<\/h6>\n<ul>\n<li>The transgene cloned into a MosSCI targeting vector for the <em>ttTi5605<\/em> site.<\/li>\n<li>A strain containing the universal MosSCI landing site.<\/li>\n<\/ul>\n<h6>Schematic of method<\/h6>\n<p><a href=\"http:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/universal-minimos1.png\"><img loading=\"lazy\" decoding=\"async\" data-attachment-id=\"482\" data-permalink=\"https:\/\/wormbuilder.org\/old\/?attachment_id=482\" data-orig-file=\"https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/universal-minimos1.png\" data-orig-size=\"627,734\" data-comments-opened=\"0\" data-image-title=\"universal-minimos\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/universal-minimos1.png\" class=\"aligncenter size-full wp-image-482\" alt=\"universal-minimos\" src=\"http:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/universal-minimos1.png\" width=\"627\" height=\"734\" srcset=\"https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/universal-minimos1.png 627w, https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/universal-minimos1-256x300.png 256w\" sizes=\"auto, (max-width: 627px) 100vw, 627px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>About universal MosSCI sites The universal MosSCI sites are insertion sites that allow insertion of the &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"more-link\" href=\"https:\/\/wormbuilder.org\/old\/?page_id=397\"> <span class=\"screen-reader-text\">Universal MosSCI<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-397","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-6p","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/397","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=397"}],"version-history":[{"count":10,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/397\/revisions"}],"predecessor-version":[{"id":469,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/397\/revisions\/469"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=397"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}