{"id":394,"date":"2013-06-19T23:18:55","date_gmt":"2013-06-19T23:18:55","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=394"},"modified":"2013-06-21T14:36:10","modified_gmt":"2013-06-21T14:36:10","slug":"minimos","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=394","title":{"rendered":"miniMos"},"content":{"rendered":"<h4><span style=\"font-size: 1em;\">About the miniMos transposon<\/span><\/h4>\n<p>The miniMos transposon is a method to carry transgenes into the\u00a0<em>C. elegans<\/em>\u00a0genome. \u00a0The method has the advantages:<\/p>\n<ul>\n<li>The insertion frequency and fidelity is high<\/li>\n<li>The exact insertion site can be determined<\/li>\n<li>Large transgenes can be inserted<\/li>\n<li>The miniMos element is active in\u00a0<em>C. elegans<\/em>,\u00a0<em>C. briggsae<\/em>\u00a0and natural isolates.<\/li>\n<li>Transgenes are expressed in the germline at high frequency.<\/li>\n<\/ul>\n<p>The method works by inserting a transgene into a modified Mos1 element (miniMos). The miniMos transposon can carry the inserted transgene (together with a selection marker) into the genome. Insertions are generated directly by injecting worms and take approximately 1 week to generate. The insertion site is random and can be identified by inverse PCR on the strain.<\/p>\n<h6>Requirements<\/h6>\n<ul>\n<li>The transgene cloned into the appropriate miniMos vector (different selection markers)<\/li>\n<\/ul>\n<h6>Schematic of method<\/h6>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter\" alt=\"miniMOS\" src=\"http:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/06\/miniMOS.png\" width=\"627\" height=\"687\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>About the miniMos transposon The miniMos transposon is a method to carry transgenes into the\u00a0C. elegans\u00a0genome. &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"more-link\" href=\"https:\/\/wormbuilder.org\/old\/?page_id=394\"> <span class=\"screen-reader-text\">miniMos<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-394","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-6m","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/394","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=394"}],"version-history":[{"count":5,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/394\/revisions"}],"predecessor-version":[{"id":515,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/394\/revisions\/515"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=394"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}