{"id":36,"date":"2013-02-20T14:48:14","date_gmt":"2013-02-20T14:48:14","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=36"},"modified":"2013-03-21T10:18:50","modified_gmt":"2013-03-21T10:18:50","slug":"strains","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=36","title":{"rendered":"Insertion strains"},"content":{"rendered":"\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Locus<\/strong><\/th>Genetic position<\/strong><\/th>Chromosomal Position<\/strong><\/th>Strain<\/strong><\/th>Germline expression<\/strong><\/th>CGC<\/strong><\/th>\n<\/tr>\n<\/thead>\n
unc-119<\/em><\/strong><\/td><\/td><\/td><\/td><\/td><\/td>\n<\/tr>\n
ttTi4348<\/em><\/td>I:-5.32<\/td>I: 2.85 MB<\/td>EG6701<\/td>Yes<\/td>Request strain<\/a><\/td>\n<\/tr>\n
ttTi4391<\/em><\/td>I:7.93<\/td>I: 11.27 MB<\/td>EG6702<\/td>No<\/td>Request strain<\/a><\/td>\n<\/tr>\n
ttTi5605<\/em><\/td>II:0.77<\/td>II: 8.42 MB<\/td>EG6699<\/td>Yes<\/td>Request strain<\/a><\/td>\n<\/tr>\n
cxTi10816<\/em><\/td>IV:1.41<\/td>IV: 5.01 MB<\/td>EG6703<\/td>Yes<\/td>Request strain<\/a><\/td>\n<\/tr>\n
cxTi10882<\/em><\/td>IV:-0.05<\/td>IV: 4.24 MB<\/td>EG6700<\/td>Yes (variable)<\/td>Request strain<\/a><\/td>\n<\/tr>\n
ttTi14024<\/em><\/td>X:22.84<\/td>X: 15.57 MB<\/td>EG6705<\/td>Late stages only<\/td>Request strain<\/a><\/td>\n<\/tr>\n
unc-18<\/em><\/strong><\/td><\/td><\/td><\/td><\/td><\/td>\n<\/tr>\n
ttTi4348<\/em><\/td>I:-5.32<\/td>I: 2.85 MB<\/td>EG6032<\/td>Yes<\/td>Request strain<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

Most strains were injected with a rescuing array containing a bright GFP and cb-unc-119<\/em> to facilitate freezing and strain handling. It is necessary<\/strong> to loose the array before injecting worms to generate MosSCI insertions. <\/p>\n","protected":false},"excerpt":{"rendered":"

Most strains were injected with a rescuing array containing a bright GFP and cb-unc-119 to facilitate …<\/p>\n

Insertion strains<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":23,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-36","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-A","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/36","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=36"}],"version-history":[{"count":6,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/36\/revisions"}],"predecessor-version":[{"id":277,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/36\/revisions\/277"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/23"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=36"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}