{"id":315,"date":"2013-05-09T09:57:03","date_gmt":"2013-05-09T09:57:03","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=315"},"modified":"2014-02-23T21:31:22","modified_gmt":"2014-02-24T03:31:22","slug":"laco-insertions","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=315","title":{"rendered":"LacO insertions"},"content":{"rendered":"

Use search box to limit strains to chromosomes (LG1, LG2, LG3, LG4, LG5 or LGX) or resistance marker (PuroR, NeoR).<\/em><\/p>\n

Each strain has been tested for antibiotic resistance but not for LacI binding. Please note that the lacO<\/em> insertions were put into a strain with the syIs46<\/em> insertion. This insertion has both LacI-GFP but also\u00a0lacO<\/em>\u00a0sites. The brightest spot present in these strains are therefore from the syIs46<\/em> array integration and the dimmer spot from the 256x lacO<\/em>\u00a0miniMos insertion (thank you to Peter Meister<\/a> for clarifying this). It is highly recommended to outcross 256x lacO<\/em>\u00a0from syIs46<\/em> for use as a chromosomal marker.<\/p>\n

Please request strains from the CGC<\/a>.
\nStrain column has link to the strain info at the CGC.<\/p>\n

If you find the strains useful then please cite\u00a0“Fr\u00f8kj\u00e6r-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella M, Pozniakovski A, Moerman DG and Jorgensen EM. Random and targeted transgene insertion in\u00a0C. elegans<\/em>\u00a0using a modified Mos1 transposon.\u00a0Nature Methods<\/em>. (In press)”.<\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Chr.<\/th>Genetic pos.<\/th>Position (WB230)<\/th>Cargo<\/th>Allele<\/th>Strain<\/th>Insertion marker(s)<\/th>Genotype<\/th>Flanking sequence<\/th>Comments<\/th>\n<\/tr>\n<\/thead>\n
LG3<\/td>III:-26.98<\/td>118,056<\/td>256x LacO and NeoR<\/td>oxTi467<\/em><\/td>EG7522<\/a><\/td>cb-unc-119(+)<\/em> and NeoR<\/td>syIs46 II; unc-119(ed3)<\/em> III oxTi467 III<\/td>TACATCATAGCCTGATCCT<\/td><\/td>\n<\/tr>\n
LG1<\/td>I:-19.15<\/td>147,105<\/td>256x LacO and NeoR<\/td>oxTi468<\/em><\/td>EG7523<\/a><\/td>cb-unc-119(+)<\/em> and NeoR<\/td>oxTi468 I ; syIs46 II; unc-119(ed3)<\/em> III<\/td>TATCTTTGTCACTCAGACGTTCCACAAGTT<\/td><\/td>\n<\/tr>\n
LG4<\/td>IV:14.38<\/td>15,809,942<\/td>256x LacO and NeoR<\/td>oxTi469<\/em><\/td>EG7524<\/a><\/td>cb-unc-119(+)<\/em> and NeoR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi469 IV<\/td>TAAGATTTAATTGTTAAAAAAAAGCCCTAA<\/td><\/td>\n<\/tr>\n
LG2<\/td>II:-0.39<\/td>6,267,597<\/td>256x LacO and PuroR<\/td>oxTi470<\/em><\/td>EG7525<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi470 II syIs46 II; unc-119(ed3)<\/em> III<\/td>TATTTGTCAACTGAAATTTATCATTTTTTA<\/td><\/td>\n<\/tr>\n
LG2<\/td>II:10.59<\/td>12,635,479<\/td>256x LacO and PuroR<\/td>oxTi471<\/em><\/td>EG7526<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi471 II ; syIs46 II; unc-119(ed3)<\/em> III<\/td>TAGAACTTCAAGACGCGAACCTTGAAAAAA<\/td><\/td>\n<\/tr>\n
LG2<\/td>II:24.11<\/td>15,142,879<\/td>256x LacO and PuroR<\/td>oxTi472<\/em><\/td>EG7527<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi472 II ; syIs46 II; unc-119(ed3)<\/em> III<\/td>TAATGTATTTTAATACAGAATAGTCTCGAG<\/td><\/td>\n<\/tr>\n
LG2<\/td>II:-0.58<\/td>6,074,479<\/td>256x LacO and PuroR<\/td>oxTi473<\/em><\/td>EG7528<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi473 II ; syIs46 II; unc-119(ed3)<\/em> III<\/td>TAATATGACAATTAGTAGTCATATGGATGC<\/td><\/td>\n<\/tr>\n
LG5<\/td>V:24.97<\/td>20,209,942<\/td>256x LacO and PuroR<\/td>oxTi474<\/em><\/td>EG7529<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II ; unc-119(ed3)<\/em> III ; oxTi474 IV<\/td>TAAATGATTGAACAGATTCTTTGTTTTGAA<\/td><\/td>\n<\/tr>\n
LG5<\/td>V:-19.85<\/td>1,245,918<\/td>256x LacO and PuroR<\/td>oxTi475<\/em><\/td>EG7530<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi475 V<\/td>TATATGTACATATTATTCTCATAAATCTCA<\/td><\/td>\n<\/tr>\n
LG5<\/td>V:2.83<\/td>10,899,001<\/td>256x LacO and PuroR<\/td>oxTi476<\/em><\/td>EG7531<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi476 V<\/td>TATTACTTTTCTAAAGTTTTCAAGAATAAA<\/td><\/td>\n<\/tr>\n
LG4<\/td>IV:3.59<\/td>8,033,901<\/td>256x LacO and PuroR<\/td>oxTi477<\/em><\/td>EG7532<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi477 IV<\/td>TACATAAAAAATACTCATTGATTAATTGGT<\/td><\/td>\n<\/tr>\n
LG5<\/td>V:4.62<\/td>12,728,334<\/td>256x LacO and PuroR<\/td>oxTi478<\/em><\/td>EG7533<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi478 V<\/td>TAATACAAAAAATTCGATATTTTACTCAAA<\/td><\/td>\n<\/tr>\n
LG1<\/td>I:-21.83<\/td>4,915<\/td>256x LacO and PuroR<\/td>oxTi479<\/em><\/td>EG7541<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi479 I ; syIs46 II; unc-119(ed3)<\/em> III<\/td>TACTACACGTGGGCAAGTTTATACAGTTTT<\/td><\/td>\n<\/tr>\n
LG2<\/td>II:-5.64<\/td>4,063,707<\/td>256x LacO and PuroR<\/td>oxTi480<\/em><\/td>EG7534<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi480 II syIs46 II; unc-119(ed3)<\/em> III<\/td>TATCTTATCGTATGAATTGTGTCCGTTTCT<\/td><\/td>\n<\/tr>\n
LG1<\/td>I:2.10<\/td>7,530,607<\/td>256x LacO and PuroR<\/td>oxTi481<\/em><\/td>EG7535<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>oxTi481 I ; syIs46 II; unc-119(ed3)<\/em> III<\/td>TAATTGTTGGAGCAATTGTTGTCCTTGGTT<\/td><\/td>\n<\/tr>\n
LG4<\/td>IV:4.03<\/td>9,080,905<\/td>256x LacO and PuroR<\/td>oxTi482<\/em><\/td>EG7536<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi482 IV<\/td>TAAATAATTTGATTCTTTTAAGACAATTAT<\/td><\/td>\n<\/tr>\n
LGX<\/td>X:7.04<\/td>12,405,642<\/td>256x LacO and PuroR<\/td>oxTi483<\/em><\/td>EG7477<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi483 X<\/td>TATCCAGCCCGACGAGTCTAGTAGTGGATA<\/td><\/td>\n<\/tr>\n
LG3<\/td>III:3.96<\/td>10,613,761<\/td>256x LacO and PuroR<\/td>oxTi485<\/em><\/td>EG7538<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III Ti485 III<\/td>TATATCAATTGGGAATGCACGGAAATTTAA<\/td><\/td>\n<\/tr>\n
LG5<\/td>V:-17.21<\/td>1,810,279<\/td>256x LacO and PuroR<\/td>oxTi486<\/em><\/td>EG7539<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II; unc-119(ed3)<\/em> III ; oxTi486 V<\/td>TATGTATTAAGTTGGCCTATATACCAAGTT<\/td>This strain may be sick (sterility and lack of GFP noted by Rog lab)<\/td>\n<\/tr>\n
LGX<\/td>X:21.84<\/td>15,091,074<\/td>256x LacO and PuroR<\/td>oxTi487<\/em><\/td>EG7478<\/a><\/td>cb-unc-119(+)<\/em> and PuroR<\/td>syIs46 II ; unc-119(ed3)<\/em> III ; oxTi487 X<\/td>TAGTGAAAGAAACGCTAGGAGAAGATACTG<\/td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"

Use search box to limit strains to chromosomes (LG1, LG2, LG3, LG4, LG5 or LGX) or …<\/p>\n

LacO insertions<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-315","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-55","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/315","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=315"}],"version-history":[{"count":8,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/315\/revisions"}],"predecessor-version":[{"id":841,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/315\/revisions\/841"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=315"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}