{"id":26,"date":"2013-02-20T14:28:08","date_gmt":"2013-02-20T14:28:08","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=26"},"modified":"2013-03-21T09:53:45","modified_gmt":"2013-03-21T09:53:45","slug":"plasmids","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=26","title":{"rendered":"Plasmids"},"content":{"rendered":"

MosSCI<\/h3>\n

Co-injection markers<\/h5>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Plasmid<\/th>Description<\/th>Transgene<\/th>ApE file<\/th>\"Addgene<\/th>\n<\/tr>\n<\/thead>\n
pCFJ601<\/strong><\/td>Induces Mos1 transposition by injection.<\/td>Peft-3<\/em>:Mos1 transposase<\/td>pCFJ601<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pJL44<\/strong><\/td>Induces Mos1 transposition by heat-shock.<\/td>Phsp-16.48<\/em>:Mos1 transposase<\/td>pJL44<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pMA122<\/strong><\/td>Negative selection, heat-shock inducible.<\/td>Phsp-16.41<\/em>:peel-1<\/em>:tbb-2<\/em> UTR<\/td>pMA122<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pGH8<\/strong><\/td>Visual extra-chromosomal array marker. Red, nervous system, cytosolic.<\/td>Prab-3<\/em>:mCherry:unc-54<\/em> UTR<\/td>pGH8<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ90<\/strong><\/td>Visual extra-chromosomal array marker. Red, pharynx, cytosolic. <\/td>Pmyo-2<\/em>:mCherry:unc-54<\/em> UTR<\/td>pCFJ90<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ104<\/strong><\/td>Visual extra-chromosomal array marker. Red, body wall muscle, cytosolic.<\/td>Pmyo-3<\/em>:mCherry:unc-54<\/em> UTR<\/td>pCFJ104<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ420<\/strong><\/td>Visual extra-chromosomal array marker. Green, ubiquitous, nuclear. <\/td>Peft-3<\/em>:GFP:H2B:tbb-2<\/em> UTR<\/td>pCFJ420<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ421<\/strong><\/td>Visual extra-chromosomal array marker. Green, pharynx, nuclear.<\/td>Pmyo-2<\/em>:GFP:H2B:tbb-2<\/em> UTR<\/td>pCFJ421<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n
Site-specific MosSCI vectors<\/h5>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Plasmid<\/strong><\/th> Locus <\/strong><\/th> Genetic Position <\/strong><\/th> Chromosomal Position <\/strong><\/th>Ape File<\/th> Selection <\/strong><\/th>\"Addgene<\/a><\/th>\n<\/tr>\n<\/thead>\n
Three fragment Gateway vectors<\/strong><\/td><\/td><\/td><\/td><\/td><\/td><\/td>\n<\/tr>\n
pCFJ210<\/strong><\/td>ttTi4348<\/a><\/em><\/td> I:-5.32<\/td>I: 2.85 MB<\/td>pCFJ210<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ448<\/strong><\/td>ttTi4348<\/a><\/em><\/td> I:-5.32<\/td>I: 2.85 MB<\/td>pCFJ448<\/a><\/td>unc-18<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ604<\/strong><\/td>ttTi4391<\/a><\/em><\/td>I:7.93<\/td>I: 11.27 MB<\/td>pCFJ604<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ150<\/strong><\/td>ttTi5605<\/a><\/em><\/td>II:0.77<\/td>II: 8.42 MB<\/td>pCFJ150<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ212<\/strong><\/td>cxTi10816<\/a><\/em><\/td>IV:1.41<\/td>IV: 5.01 MB<\/td>pCFJ212<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ201<\/strong><\/td>cxTi10882<\/a><\/em><\/td>IV:-0.05<\/td>IV: 4.24 MB<\/td>pCFJ201<\/a><\/td>cb-unc-119<\/em><\/td> <\/td>\n<\/tr>\n
pCFJ606<\/strong><\/td>ttTi14024<\/a><\/em><\/td>X:22.84<\/td>X: 15.57 MB<\/td>pCFJ606<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
Multiple Cloning Site vectors<\/strong><\/td><\/td><\/td><\/td><\/td><\/td><\/td>\n<\/tr>\n
pCFJ352<\/strong><\/td>ttTi4348<\/a><\/em><\/td> I:-5.32<\/td>I: 2.85 MB<\/td>pCFJ352<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ676<\/strong><\/td>ttTi4348<\/a><\/em><\/td> I:-5.32<\/td>I: 2.85 MB<\/td>pCFJ676<\/a><\/td>unc-18<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ353<\/strong><\/td>ttTi4391<\/a><\/em><\/td>I:7.93<\/td>I: 11.27 MB<\/td>pCFJ353<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ350<\/strong><\/td>ttTi5605<\/a><\/em><\/td>II:0.77<\/td>II: 8.42 MB<\/td>pCFJ350<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ356<\/strong><\/td>cxTi10816<\/a><\/em><\/td>IV:1.41<\/td>IV: 5.01 MB<\/td>pCFJ356<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ351<\/strong><\/td>cxTi10882<\/a><\/em><\/td>IV:-0.05<\/td>IV: 4.24 MB<\/td>pCFJ351<\/a><\/td>cb-unc-119<\/em><\/td> <\/td>\n<\/tr>\n
pCFJ355<\/strong><\/td>ttTi14024<\/a><\/em><\/td>X:22.84<\/td>X: 15.57 MB<\/td>pCFJ355<\/a><\/td>cb-unc-119<\/em><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"

MosSCI Co-injection markers Site-specific MosSCI vectors<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":23,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-26","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-q","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/26","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26"}],"version-history":[{"count":10,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/26\/revisions"}],"predecessor-version":[{"id":1176,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/26\/revisions\/1176"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/23"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}