{"id":15,"date":"2013-02-20T14:23:24","date_gmt":"2013-02-20T14:23:24","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=15"},"modified":"2013-06-21T14:30:35","modified_gmt":"2013-06-21T14:30:35","slug":"test-page","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=15","title":{"rendered":"MosSCI"},"content":{"rendered":"<h4>About mos1-mediated Single Copy Insertion (mosSCI)<\/h4>\n<p>mosSCI is a method to insert a single copy of a transgene into a well-defined location in the <em>C. elegans<\/em> genome. The method has the advantages:<\/p>\n<ul>\n<li>Transgenes can be expressed at levels close to endogenous gene expression<\/li>\n<li>Transgene insertions are stable<\/li>\n<li>Transgene expression is possible in the germline.<\/li>\n<\/ul>\n<p>The method works by breaking a chromosome at a particular location by excising a Mos1 transposon. The excision creates a double-strand DNA break that is repaired by the cell. In the presence of a DNA template with homology to the breakpoint then the repair process will incorporate DNA from the repair template into the genome. We have incorporated a positive selection marker to select for this event (typically <em>unc-119<\/em>) and fluorescent markers to identify extra-chromosomal arrays.<\/p>\n<h6>Requirements<\/h6>\n<ul>\n<li><span style=\"line-height: 13px;\">A transgene cloned into the appropriate targeting vector.\u00a0<\/span><\/li>\n<li>A strain containing a Mos1 transposon at the site corresponding to the targeting vector.<\/li>\n<\/ul>\n<h6>Schematic of method<\/h6>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" data-attachment-id=\"486\" data-permalink=\"https:\/\/wormbuilder.org\/old\/?attachment_id=486\" data-orig-file=\"https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/02\/MosSCI1.png\" data-orig-size=\"627,734\" data-comments-opened=\"0\" data-image-title=\"MosSCI\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/02\/MosSCI1.png\" class=\"aligncenter size-full wp-image-486\" alt=\"MosSCI\" src=\"http:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/02\/MosSCI1.png\" width=\"627\" height=\"734\" srcset=\"https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/02\/MosSCI1.png 627w, https:\/\/wormbuilder.org\/old\/wp-content\/uploads\/2013\/02\/MosSCI1-256x300.png 256w\" sizes=\"auto, (max-width: 627px) 100vw, 627px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>About mos1-mediated Single Copy Insertion (mosSCI) mosSCI is a method to insert a single copy of &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"more-link\" href=\"https:\/\/wormbuilder.org\/old\/?page_id=15\"> <span class=\"screen-reader-text\">MosSCI<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-15","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-f","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/15","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=15"}],"version-history":[{"count":10,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/15\/revisions"}],"predecessor-version":[{"id":513,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/15\/revisions\/513"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=15"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}