{"id":1236,"date":"2017-02-08T16:09:07","date_gmt":"2017-02-08T22:09:07","guid":{"rendered":"http:\/\/wormbuilder.org\/old\/?page_id=1236"},"modified":"2017-02-08T16:51:33","modified_gmt":"2017-02-08T22:51:33","slug":"codon-optimized-flourophores","status":"publish","type":"page","link":"https:\/\/wormbuilder.org\/old\/?page_id=1236","title":{"rendered":"Codon-optimized flourophores"},"content":{"rendered":"

Plasmids described in Froekjaer-Jensen et al (2016)<\/a>. In general, the C. elegans codon optimized fluorophores are better expressed in the germline, especially when the coding region contains PATCs.<\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t
Plasmid<\/th>Description<\/th>Transgene<\/th>ApE file<\/th>\"Addgene<\/th>\n<\/tr>\n<\/thead>\n
pCFJ1955<\/strong><\/td>C. elegans<\/em> optimized tdTomato in expression construct in minimos vector<\/td>Peft-3<\/em>:ceTdTomato(2xNLS):tbb-2<\/em> UTR<\/td>pCFJ1955 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ce-tdTomato(syntrons, NLS) | tbb-2 3UTR].ape<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1320<\/strong><\/td>C. elegans<\/em> optimized GFP in expression construct in minimos vector<\/td>Peft-3<\/em>:ceGFP(2xNLS):tbb-2<\/em> UTR<\/td>pCFJ1320 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(smu-2 introns) | tbb-2 3UTR].ape<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1415<\/strong><\/td>C. elegans<\/em> optimized GFP with PATCs<\/strong> in expression construct in minimos vector<\/td>Peft-3<\/em>:ceGFP(PATC, 2xNLS):tbb-2<\/em> UTR<\/td>pCFJ1415 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(smu-1 introns) | tbb-2 3UTR].ape<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ1137<\/strong><\/td>C. elegans<\/em> optimized GFP in expression construct in minimos vector<\/td>Peft-3<\/em>:ceGFP(2xNLS):tbb-2<\/em> UTR<\/td>pCFJ1137 - pEXP(ttTi5605, cbr-unc-119)[Peft-3 | ceGFP(synthetic introns) | tbb-2 3UTR.ape<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n
pCFJ889<\/strong><\/td>GFP tagged smu-1<\/em> gene in minimos vector<\/td>Psmu-1:smu-1:gfp:smu-1<\/em>UTR<\/td>pCFJ889 - pEXP(minimos, cbr-unc-119)[Psmu-1 | smu-1| GFP-smu-1 3UTR].ape<\/a><\/td>Request plasmid<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Plasmids described in Froekjaer-Jensen et al (2016). In general, the C. elegans codon optimized fluorophores are …<\/p>\n

Codon-optimized flourophores<\/span> Read more \u2192<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":23,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-1236","page","type-page","status-publish","hentry"],"jetpack_shortlink":"https:\/\/wp.me\/P3eUbV-jW","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/1236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1236"}],"version-history":[{"count":4,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/1236\/revisions"}],"predecessor-version":[{"id":1251,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/1236\/revisions\/1251"}],"up":[{"embeddable":true,"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=\/wp\/v2\/pages\/23"}],"wp:attachment":[{"href":"https:\/\/wormbuilder.org\/old\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}